Detects human Cytosolic Sulfotransferase 1A1/SULT1A1 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) SULT1A3 and rhSULT1C4 and approximately 10% cross-reactivity with rhSULT1B1 is observed. No cross-reactivity with rhSULT1E1, 2A1, 2B1, or 4A1 is observed.
Monoclonal Mouse IgG1 Clone # 638708
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Cytosolic Sulfotransferase 1A1/SULT1A1 Glu2-Leu295 Accession # P50225
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human Cytosolic Sulfotransferase 1A1/SULT1A1 by Western Blot.
Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 0.5 µg/mL of Human Cytosolic Sulfotransferase 1A1/SULT1A1 Monoclonal Antibody (Catalog # MAB5546) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cytosolic Sulfotransferase 1A1/SULT1A1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cytosolic Sulfotransferase 1A1/SULT1A1 in Human Brain.
Cytosolic Sulfotransferase 1A1/SULT1A1 was detected in immersion fixed paraffin-embedded sections of human brain using Human Cytosolic Sulfotransferase 1A1/SULT1A1 Monoclonal Antibody (Catalog # MAB5546) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons and glial cells (punctate). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Cytosolic Sulfotransferase 1A1 (SULT1A1; also phenol sulfotransferase 1 and thermostable phenol sulfotransferase) is a 35 kDa enzyme belonging to the sulfotransferase 1 family of proteins. Human SULT1A1 is 295 amino acids (aa) in length. Human SULT1A1 shares 78% and 73% aa identity with rat and mouse SULT1A1, respectively. SULT1A1 exists as a homodimer and is expressed in liver, lung, adrenal gland, brain, platelets, and skin. Functionally, it catalyzes the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitter. It also mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could participate as a modulating factor of cancer risk.
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