|Detection of Human Cytosolic Sulfotransferase 1A1/SULT1A1 by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 0.5 µg/mL of Human Cytosolic Sulfotransferase 1A1/SULT1A1 Monoclonal Antibody (Catalog # MAB5546) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cytosolic Sulfotransferase 1A1/SULT1A1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
|Cytosolic Sulfotransferase 1A1/SULT1A1 in Human Brain. Cytosolic Sulfotransferase 1A1/SULT1A1 was detected in immersion fixed paraffin-embedded sections of human brain using Human Cytosolic Sulfotransferase 1A1/SULT1A1 Monoclonal Antibody (Catalog # MAB5546) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons and glial cells (punctate). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.|
Cytosolic Sulfotransferase 1A1 (SULT1A1; also phenol sulfotransferase 1 and thermostable phenol sulfotransferase) is a 35 kDa enzyme belonging to the sulfotransferase 1 family of proteins. Human SULT1A1 is 295 amino acids (aa) in length. Human SULT1A1 shares 78% and 73% aa identity with rat and mouse SULT1A1, respectively. SULT1A1 exists as a homodimer and is expressed in liver, lung, adrenal gland, brain, platelets, and skin. Functionally, it catalyzes the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitter. It also mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could participate as a modulating factor of cancer risk.
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