Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 656229
View all Cytosolic Sulfotransferase 2A1/SULT2A1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Cytosolic Sulfotransferase 2A1/SULT2A1
Ser2-Glu285
Accession # NP_003158

Specificity

Detects human Cytosolic Sulfotransferase 2A1/SULT2A1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human SULT1A1, 1A3, 4A1, 1B1, 2B1, or 1E1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody

Detection of Human Cytosolic Sulfotransferase 2A1/SULT2A1 antibody by Western Blot.

Detection of Human Cytosolic Sulfotransferase 2A1/SULT2A1 by Western Blot.

Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Cytosolic Sulfotransferase 2A1/ SULT2A1 Mono-clonal Antibody (Catalog # MAB5828) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cytosolic Sulfotransferase 2A1/SULT2A1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cytosolic Sulfotransferase 2A1/SULT2A1 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

Cytosolic Sulfotransferase 2A1/SULT2A1 in HepG2 Human Cell Line.

Cytosolic Sulfotransferase 2A1/SULT2A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Cytosolic Sulfotransferase 2A1/ SULT2A1 Monoclonal Antibody (Catalog # MAB5828) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Western Blot

2 µg/mL
Sample: Human liver tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Cytosolic Sulfotransferase 2A1/SULT2A1

Sulfotransferase 2A1 (SULT2A1), also called DHEA-ST (DHEA/bile acid sulfotransferase), is a 35 kDa member of the cytosolic sulfotransferase family, which are Phase II drug-metabolizing enzymes that catalyze the sulfation of many endogenous and xenobiotic substrates. SULT2A1 is primarily expressed in liver and adrenal tissues. It sulfates steroids and bile acids, playing roles in maintaining cholesterol and bile acid homeostasis by increasing cholesterol catabolism and preventing toxicity from bile acid accumulation. Human SULT2A1 shares 63-65% amino acid identity with mouse and rat SULT2A1.

Alternate Names

DHEA-ST, DHEAS, hSTa, ST2A3, SULT2A1

Entrez Gene IDs

6822 (Human); 20859 (Mouse); 24912 (Rat)

Gene Symbol

SULT2A1

UniProt

NP_003158

Additional Cytosolic Sulfotransferase 2A1/SULT2A1 Products

Product Documents for Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody

For research use only

Customer Reviews for Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody

There are currently no reviews for this product. Be the first to review Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody and earn rewards!

Have you used Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies