Human DAB2 Antibody

Catalog # Availability Size / Price Qty
AF8064
AF8064-SP
Detection of Human DAB2 by Western Blot.
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Human DAB2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DAB2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human DAB2
Lys630-Ala770
Accession # P98082
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human DAB2 by Western Blot. View Larger

Detection of Human DAB2 by Western Blot. Western blot results for A172, HeLa and MCF 10A cell line lysates. A PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human DAB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8064) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DAB2 at approximately 96 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry DAB2 in A172 Human Cell Line. View Larger

DAB2 in A172 Human Cell Line. DAB2 was detected in immersion fixed A172 cells using Goat Anti-Human DAB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8064) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized in the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry DAB2 in Human Prostate. View Larger

DAB2 in Human Prostate. DAB2 was detected in formalin fixed paraffin-embedded sections of human prostate using Goat Anti-Human DAB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8064) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DAB2

DAB2 (Drosophila disabled homolog 2; also DOC-2/Differentially-expressed in Ovarian Carcinoma and p96) is a cytoplasmic phosphoprotein. Although its predicted MW is 82 kDa, it runs anomalously at 95-105 kDa in SDS-PAGE. It is widely expressed, being found in ovarian cuboidal epithelium, megakaryocytes, fibroblasts, macrophages, breast epithelium and renal proximal tubule cells. Reduced expression levels are associated with tumor development. DAB2 has multiple functions, but is typically described as an adaptor protein (i.e.-one that supports the apposition of two interacting molecules) involved in intracellular trafficking. Molecules reported to interact with DAB2 include megalin, SMAD2 plus TGF-beta RI/II, axin and Dvl3. With respect to trafficking, DAB2 reportedly binds to AP-2, clathrin, LRP6 and myosin VI, four molecules associated with the endocytic process. Human DAB2 is 770 amino acids (aa) in length. It contains one pleckstrin homology-like domain that binds phosphotyrosine (aa 42-170) and a C-terminal Pro-rich region that binds SH3 domains (aa 600-630). There are two isoform variants. One is 80-82 kDa in size and shows a deletion of aa 230-447. The second exhibits a short deletion of aa 209-229. Over aa 630-770, human DAB2 shares 76% aa sequence identity with mouse DAB2.

Long Name
Disabled Homolog 2, Mitogen-Responsive Phosphoprotein
Entrez Gene IDs
1601 (Human); 13132 (Mouse); 79128 (Rat)
Alternate Names
DAB2; Differentially-expressed protein 2; disabled (Drosophila) homolog 2 (mitogen-responsive phosphoprotein); disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila); Disabled-2; DOC2; DOC-2DOC2disabled homolog 2; FLJ26626; mitogen-responsive phosphoprotein

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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