Human DC-LAMP Lumenal Domain Antibody

R&D Systems | Catalog # AF4087

R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DC-LAMP Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DC‑LAMP Lumenal Domain
Asp21-Thr381
Accession # EAW78337

Specificity

Detects human DC‑LAMP Lumenal Domain in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human LAMP is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human DC-LAMP Lumenal Domain Antibody

Detection of DC‑LAMP antibody in Human Mature Dendritic Cells antibody by Flow Cytometry.

Detection of DC‑LAMP in Human Mature Dendritic Cells by Flow Cytometry.

Human mature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14+cells treated with 20 ng/mL Recombinant Human IL-4 (204-IL) and 50 ng/mL Recombinant Human GM-CSF (215-GM) for 7 days and 1 µg/mL LPS, 20 ng/mL Recombinant Human TNF-a (210-TA), and 20 ng/mL Recombinant Human IL-1 beta /IL-1F2 (201-LB) for last 24 hours were stained with Goat Anti-Human DC-LAMP Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4087, filled histogram) or isotype control (AB-108-C, open histogram), followed by Donkey Anti-Goat IgG (H+L) Allophycocyanin (F0108).

Applications for Human DC-LAMP Lumenal Domain Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human mature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14+ cells treated with Recombinant Human IL‑4 (Catalog # 204-IL), Recombinant Human GM-CSF (Catalog # 215-GM), LPS, Recombinant Human TNF‑ alpha (Catalog # 210-TA), and Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB)

Western Blot

0.1 µg/mL
Sample: Recombinant Human DC-LAMP Lumenal Domain

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DC-LAMP

DC-LAMP (dendritic cell lysosome-associated membrane protein: also known as CD208 and LAMP3) is a 50‑70 kDa member of the LAMP family of proteins. Mature human DC-LAMP is a 389 amino acid (aa) type I transmembrane protein. It has a 254 aa lumenal N‑terminus and a short 14 aa cytoplasmic tail. The molecule is found in type II pneumocytes, interdigitating DC, and various tumors. DC-LAMP serves as a marker of human mature DC, and it may play a role in normal lysosome and endosome function. Over the region used as immunogen, human DC-LAMP shares 72% and 55% aa sequence identity with canine and mouse DC-LAMP, respectively.

Long Name

Dendritic Cell-lysosome-associated Membrane Glycoprotein

Alternate Names

CD208, DCLAMP, LAMP3, TSC403

Entrez Gene IDs

27074 (Human); 239739 (Mouse); 303801 (Rat)

Gene Symbol

LAMP3

UniProt

EAW78337

Additional DC-LAMP Products

Product Documents for Human DC-LAMP Lumenal Domain Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human DC-LAMP Lumenal Domain Antibody

For research use only

Related Research Areas

Cancer Biomarkers
Lysosomal Enzymes

Citations for Human DC-LAMP Lumenal Domain Antibody

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