Human DDX17 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human DDX17 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human DDX17
    Asn498-Tyr638
    Accession # Q92841
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunocytochemistry
    1-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human DDX17 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, HEK293 human embryonic kidney cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human DDX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4530) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DDX17 at approximately 86 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
DDX17 in A431 Human Cell Line. DDX17 was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human DDX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4530) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
DDX17 in Human Liver Cancer Tissue. DDX17 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Goat Anti-Human DDX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4530) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DDX17

DDX17 is a member of the DEAD box family of proteins, a family of RNA helicases characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). DDX17 plays an essential role in many aspects of cellular RNA metabolism, including RNA transport, transcription, ribosome assembly, spliceosome function, RNA degradation, and the initiation of translation. Distribution patterns suggests that some members of this family may be involved in spermatogenesis, embryogenesis, and cellular growth and division. Alternative splicing of the DDX17 gene generates two transcript variants encoding isoforms p72 and p82. p82 is translated from a non-AUG start codon 243 bp upstream of the first in frame AUG initiator codon for p72. DDX17 is more closely related to DDX5 than to any other member of the DEAD box family.

  • Long Name:
    DEAD Box Protein 17
  • Entrez Gene IDs:
    10521 (Human); 67040 (Mouse); 315133 (Rat)
  • Alternate Names:
    DDX17; DEAD (Asp-Glu-Ala-Asp) box polypeptide 17; DEAD box protein 17; DEAD box protein p72; DEAD/H box 17; DKFZp761H2016; EC 3.6.1; EC 3.6.4.13; p72; P72DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 17 (72kD); probable ATP-dependent RNA helicase DDX17; RH70; RNA-dependent helicase p72
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