Human Decorin Antibody

Catalog #: AF143
20 Citations Datasheet / COA / SDS

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AF143
AF143-SP

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Detection of Human Decorin by Western Blot.

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Product Details

Citations (20)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Decorin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Decorin in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Decorin
Gly17-Lys359
Accession # P07585

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Simple Western

5 µg/mL

See below

Immunohistochemistry

10-20 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Decorin by Western Blot. Western blot shows lysates of human uterus tissue and human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Decorin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Decorin in Human Breast. Decorin was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; (CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to intralobular stromal tissue. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western

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Detection of Human Decorin by Simple Western^TM. Simple Western lane view shows lysates of human uterus and human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Decorin at approximately 140-149 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Decorin

Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions which flank the central region containing 10‑12 tandem leucine-rich repeats (LRR) (1, 2). The human Decorin cDNA encodes a 359 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide. The 329 aa mature protein contains twelve LRR. Alternate splicing generates five isoforms with variable length deletions (3). Mature human and mouse Decorin share 80% aa sequence identity. In Decorin, serine 34 in the N-terminal domain is O-glycosylated. Naturally occurring Decorin proteoglycan has a molecular mass of approximately 100 kDa, and the deglycosylated Decorin core protein has a mass of approximately 40 kDa. Decorin binds to fibronectin, TGF-beta, and type I and type II collagens. The binding of Decorin to various molecules was reported to be mediated via the core protein. Decorin has been implicated in matrix assembly and has also been reported to suppress the growth of various tumor cell lines by activating the epidermal growth factor receptor.

References

Naito, Z. (2005) J. Nippon Med. Sch. 72:137.
Matsushima, N. et al. (2005) Cell. Mol. Life Sci. 62:2771.
Danielson, K. et al. (1993) Genomics 15:146.

Entrez Gene IDs

1634 (Human); 13179 (Mouse)

Alternate Names

Bone proteoglycan II; CSCD; DCN; decorin proteoglycan; Decorin; dermatan sulphate proteoglycans II; DSPG2; PG40; PGII; PG-II; PGS2; PG-S2; proteoglycan core protein; SLRR1B; SLRR1BPGS2; small leucine-rich protein 1B

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Decorin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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Reciprocal interplay between asporin and decorin: Implications in gastric cancer prognosis
Authors: D Basak, Z Jamal, A Ghosh, PK Mondal, P Dey Talukd, S Ghosh, B Ghosh Roy, R Ghosh, A Halder, A Chowdhury, GK Dhali, BK Chattopadh, ML Saha, A Basu, S Roy, C Mukherjee, NK Biswas, U Chatterji, S Datta
PLoS ONE, 2021;16(8):e0255915.
Species: Human
Sample Types: Tissue Homogenaets
Applications: Western Blot
CHPF Regulates the Aggressive Phenotypes of Hepatocellular Carcinoma Cells via the Modulation of the Decorin and TGF-&beta Pathways
Authors: CH Liu, BR Wu, YJ Ho, YH Chu, WC Hsu, TJ Tseng, JP Li, WC Liao
Cancers, 2021;13(6):.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases
Authors: AF Asega, MC Menezes, D Trevisan-S, D Cajado-Car, L Bertholim, AK Oliveira, A Zelanis, SMT Serrano
Sci Rep, 2020;10(1):12912.
Species: Mouse
Sample Types: Cell Lysates, Tissue Homogenates
Applications: Western Blot
Absence of the proteoglycan decorin reduces glucose tolerance in overfed male mice
Authors: J Svärd, TH Røst, CEN Sommervoll, C Haugen, OA Gudbrandse, AE Mellgren, E Rødahl, J Fernø, SN Dankel, JV Sagen, G Mellgren
Sci Rep, 2019;9(1):4614.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Regulation of myelination by exosome associated retinoic acid release from NG2-positive cells
Authors: MB Goncalves, Y Wu, E Clarke, J Grist, C Hobbs, D Trigo, J Jack, JPT Corcoran
J. Neurosci., 2019;0(0):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-P
Osteoblasts are "educated" by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment
Authors: AD Kolb, AB Shupp, D Mukhopadhy, FC Marini, KM Bussard
Breast Cancer Res., 2019;21(1):31.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay
Adenovirus-mediated decorin expression induces cancer cell death through activation of p53 and mitochondrial apoptosis
Authors: AR Yoon, J Hong, CO Yun
Oncotarget, 2017;8(44):76666-76685.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Glucosamine exposure reduces proteoglycan synthesis in primary human endothelial cells in vitro
Food Nutr Res, 2016;60(0):32615.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot
Maternal obesity drives functional alterations in uterine NK cells
JCI Insight, 2016;1(11):e85560.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
MicroRNA 181b regulates decorin production by dermal fibroblasts and may be a potential therapy for hypertrophic scar.
Authors: Kwan P, Ding J, Tredget E
PLoS ONE, 2015;10(4):e0123054.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Probing the acceptor active site organization of the human recombinant beta1,4-galactosyltransferase 7 and design of xyloside-based inhibitors.
Authors: Saliba M, Ramalanjaona N, Gulberti S, Bertin-Jung I, Thomas A, Dahbi S, Lopin-Bon C, Jacquinet J, Breton C, Ouzzine M, Fournel-Gigleux S
J Biol Chem, 2015;290(12):7658-70.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Resistance of corneal RFUVA-cross-linked collagens and small leucine-rich proteoglycans to degradation by matrix metalloproteinases.
Authors: Zhang Y, Mao X, Schwend T, Littlechild S, Conrad G
Invest Ophthalmol Vis Sci, 2013;54(2):1014-25.
Species: Human
Sample Types: Protein
Applications: Western Blot

Serglycin is a major proteoglycan in polarized human endothelial cells and is implicated in the secretion of the chemokine GROalpha/CXCL1.
Authors: Meen AJ, Oynebraten I, Reine TM, Duelli A, Svennevig K, Pejler G, Jenssen T, Kolset SO
J. Biol. Chem., 2011;286(4):2636-47.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Effects of Ultraviolet-A and Riboflavin on the Interaction of Collagen and Proteoglycans during Corneal Cross-linking.
Authors: Zhang Y, Conrad AH, Conrad GW
J. Biol. Chem., 2011;286(15):13011-22.
Species: Bovine
Sample Types: Tissue Homogenates
Applications: Western Blot

Adenovirus-mediated gene transfer of fibromodulin inhibits neointimal hyperplasia in an organ culture model of human saphenous vein graft disease.
Authors: Ranjzad P, Salem HK, Kingston PA
Gene Ther., 2009;16(9):1154-62.
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Syndromic and non-syndromic aneurysms of the human ascending aorta share activation of the Smad2 pathway.
Authors: Gomez D, Al Haj Zen A, Borges LF, Philippe M, Gutierrez PS, Jondeau G, Michel JB, Vranckx R
J. Pathol., 2009;218(1):131-42.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr

Dermatan sulfate proteoglycan biglycan as a potential selectin L/CD44 ligand involved in selective recruitment of peripheral blood CD16(-) natural killer cells into human endometrium.
Authors: Kitaya K, Yasuo T
J. Leukoc. Biol., 2009;85(3):391-400.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot

Decorin is a secreted protein associated with obesity and type 2 diabetes.
Authors: Bolton K, Segal D, McMillan J, Jowett J, Heilbronn L, Abberton K, Zimmet P, Chisholm D, Collier G, Walder K
Int J Obes (Lond), 2008;32(7):1113-21.
Species: Human
Sample Types: Plasma
Applications: ELISA Development

Periodontitis destructions are restored by synthetic glycosaminoglycan mimetic.
Authors: Lallam-Laroye C, Escartin Q, Zlowodzki AS, Barritault D, Caruelle JP, Baroukh B, Saffar JL, Colombier ML
J Biomed Mater Res A, 2006;79(3):675-83.
Species: Hamster
Sample Types: Whole Tissue
Applications: IHC-Fr

Single-cell analysis of progenitor cell dynamics and lineage specification in the human fetal kidney.
Authors: Menon R, Otto E, Kokoruda A, Zhou J, Zhang Z, Yoon E, Chen Y, Troyanskaya O, Spence J, Kretzler M, Cebrian C
Development, 0;145(16):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC