Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Bovine, Hamster

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Immunocytochemistry, Co-IP, ELISA Development, Functional Assay, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Decorin Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Decorin
Gly17-Lys359
Accession # P07585

Specificity

Detects human Decorin in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Decorin Antibody

Detection of Human Decorin antibody by Western Blot.

Detection of Human Decorin by Western Blot.

Western blot shows lysates of human uterus tissue and human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Decorin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Decorin antibody in Human Breast by Immunohistochemistry (IHC-P).

Decorin in Human Breast.

Decorin was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; (CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to intralobular stromal tissue. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Decorin antibody by Simple WesternTM.

Detection of Human Decorin by Simple WesternTM.

Simple Western lane view shows lysates of human uterus and human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Decorin at approximately 140-149 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Human Decorin by Immunocytochemistry/ Immunofluorescence

Detection of Human Decorin by Immunocytochemistry/ Immunofluorescence

Immunohistochemical DCN expression in HSc and site-matched NS from burn patients.(a) Immunohistochemistry using a polyclonal goat anti-human DCN antibody and Alexa Fluor 488 secondary antibody (green fluorescence), and counterstained with DAPI (blue fluorescence) in representative site-matched sections of NS and HSc (scale bar = 50 μm). (b) Relative expression of DCN in matched superficial and deep NS and HSc sections was calculated from fluorescence using ImageJ (mean ± SEM, n = 4 patients, * P < 0.001). Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0123054), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Decorin by Immunocytochemistry/ Immunofluorescence

Detection of Human Decorin by Immunocytochemistry/ Immunofluorescence

Immunohistochemical DCN expression in HSc and site-matched NS from burn patients.(a) Immunohistochemistry using a polyclonal goat anti-human DCN antibody and Alexa Fluor 488 secondary antibody (green fluorescence), and counterstained with DAPI (blue fluorescence) in representative site-matched sections of NS and HSc (scale bar = 50 μm). (b) Relative expression of DCN in matched superficial and deep NS and HSc sections was calculated from fluorescence using ImageJ (mean ± SEM, n = 4 patients, * P < 0.001). Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0123054), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Decorin Antibody

Application
Recommended Usage

Immunohistochemistry

10-20 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast

Simple Western

5 µg/mL
Sample: Human uterus tissue and human heart tissue

Western Blot

0.1 µg/mL
Sample: Human uterus tissue and human heart tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Decorin

Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions which flank the central region containing 10‑12 tandem leucine-rich repeats (LRR) (1, 2). The human Decorin cDNA encodes a 359 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide. The 329 aa mature protein contains twelve LRR. Alternate splicing generates five isoforms with variable length deletions (3). Mature human and mouse Decorin share 80% aa sequence identity. In Decorin, serine 34 in the N-terminal domain is O-glycosylated. Naturally occurring Decorin proteoglycan has a molecular mass of approximately 100 kDa, and the deglycosylated Decorin core protein has a mass of approximately 40 kDa. Decorin binds to fibronectin, TGF-beta, and type I and type II collagens. The binding of Decorin to various molecules was reported to be mediated via the core protein. Decorin has been implicated in matrix assembly and has also been reported to suppress the growth of various tumor cell lines by activating the epidermal growth factor receptor.

References

  1. Naito, Z. (2005) J. Nippon Med. Sch. 72:137.
  2. Matsushima, N. et al. (2005) Cell. Mol. Life Sci. 62:2771.
  3. Danielson, K. et al. (1993) Genomics 15:146.

Alternate Names

DCN, DSPG2, PG-II, PGS2, SLRR1B

Entrez Gene IDs

1634 (Human); 13179 (Mouse)

Gene Symbol

DCN

UniProt

P07585

Additional Decorin Products

Product Documents for Human Decorin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Decorin Antibody

For research use only

Citations for Human Decorin Antibody

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Protocols

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