Human Dishevelled-1 Antibody

Catalog # Availability Size / Price Qty
AF3316
AF3316-SP
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (3)

Human Dishevelled-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Dishevelled-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) DVL‑2 and rhDVL‑3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Dishevelled-1
Arg150-Leu215
Accession # O14640
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Dishevelled‑1
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human skeletal muscle

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dishevelled-1

DVL-1 (Dishevelled-1) is a cytoplasmic phosphoprotein that is one of the three human orthologs of the Drosophila dishevelled segment polarity protein. Human DVL-1 is expressed in multiple tissues where it participates in Wnt signal transduction. DVL-1 is a modular protein containing DIX, PDZ and DEP linker regions that connect Wnt/Frizzled engagement to proteins involved in the inactivation of beta -catenin destruction in the Wnt canonical pathway. Human DVL-1 shows 93% aa identity with mouse and rat DVL-1 over the sequence used for immunization. Three shorter isoforms have been described, but it is unclear whether these are actually expressed.

Entrez Gene IDs
1855 (Human); 13542 (Mouse); 83721 (Rat)
Alternate Names
dishevelled 1 (homologous to Drosophila dsh); dishevelled, dsh homolog 1 (Drosophila); Dishevelled1; Dishevelled-1; DSH homolog 1; Dsh; DVL; DVL1; DVL1L1; MGC54245; segment polarity protein dishevelled homolog DVL-1

Product Datasheets

Citation for Human Dishevelled-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The noncanonical WNT pathway is operative in idiopathic pulmonary arterial hypertension.
    Authors: Laumanns IP, Fink L, Wilhelm J, Wolff JC, Mitnacht-Kraus R, Graef-Hoechst S, Stein MM, Bohle RM, Klepetko W, Hoda MA, Schermuly RT, Grimminger F, Seeger W, Voswinckel R
    Am. J. Respir. Cell Mol. Biol., 2009;40(6):683-91.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Dishevelled-1 Antibody

Average Rating: 3.7 (Based on 3 Reviews)

5 Star
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4 Star
66.67%
3 Star
33.33%
2 Star
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1 Star
0%

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Human Dishevelled-1 Antibody
By Anonymous on 03/11/2019
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human Dishevelled-1 Antibody
By Anonymous on 11/08/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human,Mouse

Human Dishevelled-1 Antibody
By Anonymous on 11/02/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human