Human DISP2 Antibody

Catalog # Availability Size / Price Qty
MAB8434
MAB8434-SP
Detection of Human DISP2 by Western Blot.
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Human DISP2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DISP2 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 758204
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human DISP2
Arg192-Lys477
Accession # A7MBM2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human DISP2 antibody by Western Blot. View Larger

Detection of Human DISP2 by Western Blot. Western blot shows lysates of NCI-N87 human gastric carcinoma cell line, U-118-MG human glioblastoma/astrocytoma cell line, and COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human DISP2 Monoclonal Antibody (Catalog # MAB8434) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for DISP2 at approximately 152 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DISP2

DISP2 (Dispatched Homolog 2), also known as DISPB, belongs to the dispatched family of sterol-sensing proteins.  DISP2 and the closely related DISP1 were initially identified in mice by their high homology with Dispatched, a protein required for long-range Hedgehog (Hh) signaling in Drosophila.  Like DISP1, DISP2 is a multispan (12) transmembrane protein with both cytoplasmic N- and C-terminal tails.  DISP2 contains a sterol-sensing domain (SSD) of 173 amino acids (aa), and may be involved in regulating the release of cholesterol-modified Sonic Hedgehog (Shh).  Human DISP2 is 1401 aa in length, with three predicted N-linked glycosylation sites.  Over aa 192-477, human DISP2 shares 72% and 73% aa identity with mouse and rat DISP2, respectively.

Long Name
Dispatched 2
Entrez Gene IDs
85455 (Human); 214240 (Mouse); 311324 (Rat)
Alternate Names
DISP2; Dispatched B; Dispatched Homolog 2 (Drosophila); DISPB; HsT16908; KIAA1742; Protein Dispatched Homolog 2

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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