Human DISP2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human DISP2 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 758204
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human DISP2
    Arg192-Lys477
    Accession # A7MBM2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human DISP2 by Western Blot.
Western blot shows lysates of NCI‑N87 human gastric carcinoma cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, and COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human DISP2 Monoclonal Antibody (Catalog # MAB8434) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for DISP2 at approximately 152 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DISP2
DISP2 (Dispatched Homolog 2), also known as DISPB, belongs to the dispatched family of sterol-sensing proteins.  DISP2 and the closely related DISP1 were initially identified in mice by their high homology with Dispatched, a protein required for long-range Hedgehog (Hh) signaling in Drosophila.  Like DISP1, DISP2 is a multispan (12) transmembrane protein with both cytoplasmic N- and C-terminal tails.  DISP2 contains a sterol-sensing domain (SSD) of 173 amino acids (aa), and may be involved in regulating the release of cholesterol-modified Sonic Hedgehog (Shh).  Human DISP2 is 1401 aa in length, with three predicted N-linked glycosylation sites.  Over aa 192-477, human DISP2 shares 72% and 73% aa identity with mouse and rat DISP2, respectively.
  • Long Name:
    Dispatched 2
  • Entrez Gene IDs:
    85455 (Human); 214240 (Mouse); 311324 (Rat)
  • Alternate Names:
    DISP2; Dispatched B; Dispatched Homolog 2 (Drosophila); DISPB; HsT16908; KIAA1742; Protein Dispatched Homolog 2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2B Isotype Control

Ctrl MAB0042 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG Antibody

WB AF007 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2B Antibody

Flow MAB0043  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human DISP2 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information