Western blot shows lysates of NCI‑N87 human gastric carcinoma cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, and COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human DISP2 Monoclonal Antibody (Catalog # MAB8434) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for DISP2 at approximately 152 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
DISP2 (Dispatched Homolog 2), also known as DISPB, belongs to the dispatched family of sterol-sensing proteins. DISP2 and the closely related DISP1 were initially identified in mice by their high homology with Dispatched, a protein required for long-range Hedgehog (Hh) signaling in Drosophila. Like DISP1, DISP2 is a multispan (12) transmembrane protein with both cytoplasmic N- and C-terminal tails. DISP2 contains a sterol-sensing domain (SSD) of 173 amino acids (aa), and may be involved in regulating the release of cholesterol-modified Sonic Hedgehog (Shh). Human DISP2 is 1401 aa in length, with three predicted N-linked glycosylation sites. Over aa 192-477, human DISP2 shares 72% and 73% aa identity with mouse and rat DISP2, respectively.