Human DLK2/EGFL9 Antibody Summary
Accession # Q6UY11
This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human DLK2/EGFL9 Monoclonal Antibody (Catalog # MAB97561).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Human DLK2/EGFL9 ELISA Standard Curve. Recombinant Human DLK2/EGFL9 protein was serially diluted 2-fold and captured by Mouse Anti-Human DLK2/EGFL9 Monoclonal Antibody (Catalog # MAB9756) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human DLK2/EGFL9 Monoclonal Antibody (Catalog # MAB97561) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
DLK2 (protein delta homolog 2) also called Epidermal growth factor-like protein 9 (EGFL9) is a 383 aminoacid protein with a molecular weight of 40.5 kDa. DLK2 is a single-pass type I membrane protein and is a negative regulator in the Notch pathway. DLK2 regulates adipogenesis and 2 isoforms of the human protein have been described.
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