Human DLK2/EGFL9 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB9756-100
MAB9756-SP
Human DLK2/EGFL9 ELISA Standard Curve.
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Human DLK2/EGFL9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DLK2/EGFL9 in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG1 Clone # 908812R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human embryonic kidney cell line HEK-293-derived transfected with human DLK2/EGFL9
Asp27-Ser306
Accession # Q6UY11
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human DLK2/EGFL9 Monoclonal Antibody (Catalog # MAB97561).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Human DLK2/EGFL9 ELISA Standard Curve. Recombinant Human DLK2/EGFL9 protein was serially diluted 2-fold and captured by Mouse Anti-Human DLK2/EGFL9 Monoclonal Antibody (Catalog # MAB9756) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human DLK2/EGFL9 Monoclonal Antibody (Catalog # MAB97561) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLK2/EGFL9

DLK2 (protein delta homolog 2) also called Epidermal growth factor-like protein 9 (EGFL9) is a 383 aminoacid protein with a molecular weight of 40.5 kDa. DLK2 is a single-pass type I membrane protein and is a negative regulator in the Notch pathway. DLK2 regulates adipogenesis and 2 isoforms of the human protein have been described.

Long Name
Delta-like 2 Homolog
Entrez Gene IDs
65989 (Human); 106565 (Mouse); 316232 (Rat)
Alternate Names
delta-like 2 homolog (Drosophila); DLK2; DLK-2; EGFL9; EGF-like protein 9; EGF-like-domain, multiple 9; Epidermal growth factor-like protein 9; MGC111055; MGC2487; protein delta homolog 2

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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