Human DLK2/EGFL9 Antibody

Catalog #: MAB97561 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB97561-100
MAB97561-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DLK2/EGFL9 Antibody Summary

Species Reactivity

Human

Specificity

Detects human DLK2/EGFL9 in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG_2A Clone # 908866R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human embryonic kidney cell line HEK-293-derived transfected with human DLK2/EGFL9
Asp27-Ser306
Accession # Q6UY11

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human DLK2/EGFL9 Monoclonal Antibody (Catalog # MAB9756).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human DLK2/EGFL9 ELISA Standard Curve. Recombinant Human DLK2/EGFL9 protein was serially diluted 2-fold and captured by Mouse Anti-Human DLK2/EGFL9 Monoclonal Antibody (Catalog # MAB9756) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human DLK2/EGFL9 Monoclonal Antibody (Catalog # MAB97561) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLK2/EGFL9

DLK2 (protein delta homolog 2) also called Epidermal growth factor-like protein 9 (EGFL9) is a 383 aminoacid protein with a molecular weight of 40.5 kDa. DLK2 is a single-pass type I membrane protein and is a negative regulator in the Notch pathway. DLK2 regulates adipogenesis and 2 isoforms of the human protein have been described.

Long Name

Delta-like 2 Homolog

Entrez Gene IDs

65989 (Human); 106565 (Mouse); 316232 (Rat)

Alternate Names

delta-like 2 homolog (Drosophila); DLK2; DLK-2; EGFL9; EGF-like protein 9; EGF-like-domain, multiple 9; Epidermal growth factor-like protein 9; MGC111055; MGC2487; protein delta homolog 2