Human ENPP-7/Alk-SMase Antibody

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Detection of Human ENPP‑7/Alk‑SMase by Western Blot.
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Human ENPP-7/Alk-SMase Antibody Summary

Species Reactivity
Detects human ENPP‑7/Alk‑SMase in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ENPP-2, rhENPP-5, recombinant mouse ENPP-7 is observed.
Monoclonal Mouse IgG2B Clone # 560514
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human ENPP‑7/Alk‑SMase
Accession # Q6UWV6
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ENPP-7/Alk-SMase antibody by Western Blot. View Larger

Detection of Human ENPP‑7/Alk‑SMase by Western Blot. Western blot shows lysates of human small intestine tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human ENPP-7/Alk-SMase Monoclonal Antibody (Catalog # MAB4924) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ENPP-7/Alk-SMase at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ENPP-7/Alk-SMase

ENPP-7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (Alk-SMase) is a 60 kDa GPI-linked membrane glycoprotein expressed in the intestines and in human bile. It shares 30%‑36% homology with the NPP family, but is unrelated to neutral or acid SMase. ENPP-7 hydrolyzes dietary sphingomyelin to form ceramide and phosphorylcholine, and may also hydrolyze and inactivate platelet-activating factor (PAF). It is down‑regulated in some human colorectal carcinomas. The 416 amino acid (aa) mature human ENPP-7 shares 80% and 82% aa identity with mouse and rat ENPP-7, respectively.

Long Name
Ectonucleotide Pyrophosphatase/Phosphodiesterase 7
Entrez Gene IDs
339221 (Human); 238011 (Mouse); 303729 (Rat)
Alternate Names
Alkaline sphingomyelin phosphodiesterase; alkaline sphingomyelinase; Alk-SMase; EC; ectonucleotide pyrophosphatase/phosphodiesterase 7; ectonucleotide pyrophosphatase/phosphodiesterase family member 7; E-NPP 7; ENPP7; ENPP-7; Intestinal alkaline sphingomyelinase; MGC50179; NPP7; NPP-7

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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