Detection of Human ENPP‑7/Alk‑SMase by Western Blot.
Western blot shows lysates of human small intestine tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human ENPP‑7/Alk‑SMase Monoclonal Antibody (Catalog # MAB4924) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ENPP‑7/Alk‑SMase at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
ENPP-7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (Alk-SMase) is a 60 kDa GPI-linked membrane glycoprotein expressed in the intestines and in human bile. It shares 30%‑36% homology with the NPP family, but is unrelated to neutral or acid SMase. ENPP-7 hydrolyzes dietary sphingomyelin to form ceramide and phosphorylcholine, and may also hydrolyze and inactivate platelet-activating factor (PAF). It is down‑regulated in some human colorectal carcinomas. The 416 amino acid (aa) mature human ENPP-7 shares 80% and 82% aa identity with mouse and rat ENPP-7, respectively.
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