Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 560514
View all ENPP-7/Alk-SMase Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ENPP‑7/Alk‑SMase
Pro23-Ser439
Accession # Q6UWV6

Specificity

Detects human ENPP‑7/Alk‑SMase in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ENPP-2, rhENPP-5, recombinant mouse ENPP-7 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human ENPP‑7/Alk‑SMase Antibody

Detection of Human ENPP-7/Alk-SMase antibody by Western Blot.

Detection of Human ENPP‑7/Alk‑SMase by Western Blot.

Western blot shows lysates of human small intestine tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human ENPP-7/Alk-SMase Monoclonal Antibody (Catalog # MAB4924) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ENPP-7/Alk-SMase at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human ENPP‑7/Alk‑SMase Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human small intestine tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ENPP-7/Alk-SMase

ENPP-7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (Alk-SMase) is a 60 kDa GPI-linked membrane glycoprotein expressed in the intestines and in human bile. It shares 30%‑36% homology with the NPP family, but is unrelated to neutral or acid SMase. ENPP-7 hydrolyzes dietary sphingomyelin to form ceramide and phosphorylcholine, and may also hydrolyze and inactivate platelet-activating factor (PAF). It is down‑regulated in some human colorectal carcinomas. The 416 amino acid (aa) mature human ENPP-7 shares 80% and 82% aa identity with mouse and rat ENPP-7, respectively.

Long Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 7

Alternate Names

Alk-SMase, ENPP7, NPP7

Entrez Gene IDs

339221 (Human); 238011 (Mouse); 303729 (Rat)

Gene Symbol

ENPP7

UniProt

Q6UWV6

Additional ENPP-7/Alk-SMase Products

Product Documents for Human ENPP‑7/Alk‑SMase Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ENPP‑7/Alk‑SMase Antibody

For research use only

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