Key Product Details

Species Reactivity

Validated:

Human

Cited:

Primate - Pongo (Orangutan)

Applications

Validated:

Western Blot

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 416727
View all ErbB2/Her2 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ErbB2/Her2 Herstatin Isoform
Thr23-Gly419
Accession # AAD56009

Specificity

Detects human ErbB2/Her2 Herstatin Isoform in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) EGF R or rhErbB3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human ErbB2/Her2 Herstatin Isoform Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Herstatin

Reviewed Applications

Read 1 review rated 3 using MAB4360 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ErbB2/Her2

Herstatin is a secreted 68 kDa glycoprotein encoded by an alternate transcript of the Her2/neu (ErbB2) gene containing the N-terminal 304 amino acids (aa) of subdomain I and II, and 79 intron-encoded aa at the C-terminus. It is an endogenous inhibitor of the EGF receptor family that disrupts receptor interactions and inhibits growth of tumor cells that overexpress Her2.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Entrez Gene IDs

2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)

Gene Symbol

ERBB2

UniProt

AAD56009

Additional ErbB2/Her2 Products

Product Documents for Human ErbB2/Her2 Herstatin Isoform Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ErbB2/Her2 Herstatin Isoform Antibody

For research use only

Citations for Human ErbB2/Her2 Herstatin Isoform Antibody

Customer Reviews for Human ErbB2/Her2 Herstatin Isoform Antibody (1)

3 out of 5
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  • Human ErbB2/Her2 Herstatin Isoform Antibody
    Name: Giulio Bernardinelli
    Application: Western Blot
    Sample Tested: total cell lysate from HEK293; SKBR-3; MCF-7; HeLa
    Species: Human
    Verified Customer | Posted 02/09/2018
    total cell lysate from from 300.000 cells of each of the following cell lines: HEK293; SKBR-3; MCF-7; HeLa. primary incubation 1ug/ml overnight. secondary Goat anti-mouse IRDye(R) 800CW
    Human ErbB2/Her2 Herstatin Isoform Antibody MAB4360

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