|Detection of Human ERMAP by Western Blot. Western blot shows lysates of human erythrocytes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ERMAP Monoclonal Antibody (Catalog # MAB4928) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ERMAP at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
ERMAP (erythrocyte membrane-associated protein; also Scianna blood group antigen) is a 60‑66 kDa member of the BTN/MOG family, Ig-Superfamily of proteins. It is expressed on erythrocytes and erythrocyte precursors and likely serves as a cell adhesion molecule. Mature human ERMAP is a 446 amino acid (aa) type I transmembrane glycoprotein. It possesses a 126 aa extracellular domain (ECD) (aa 30‑155) that contains one V-type Ig-like domain (aa 30‑140). Single aa changes at Gly35, Glu47, Gly57, Pro60 and Arg81 generate distinct antigens of the Scianna blood group. There is one alternate start site at Met91 and a splice variant that shows an 11 aa substitution for aa 103‑475. Over aa 30‑155, human ERMAP is 56% aa identical to mouse ERMAP. In contrast to human ERMAP ECD, mouse ERMAP ECD contains one extra Ig-like domain.
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