Detection of Human EVI‑1 by Western Blot. Western blot shows lysates of MDA‑MB‑231 human breast cancer cell line and SK‑OV‑3 human ovarian adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human EVI‑1 Monoclonal Antibody (Catalog # MAB7506) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for EVI‑1 at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Ectopic virus integration site 1 (EVI1), also known as MECOM, is a 145 kDa is a transcriptional regulator that interacts with GATA2 and histone methyltransferases. EVI1 contains 7 tandem N-terminal zinc finger regions (aa 21-239), a central region, and a cluster of 3 more zinc fingers (aa 733-812). Longer isoforms have 189 aa or 64 aa N-terminal extensions. EVI1 target genes are critical to hematopoietic stem cell proliferation and myeloid differentiation. EVI1 is overexpressed in acute myelogenous leukemia (AML) as well as ovarian cancer. Chromosomal translocations fuse EVI1 with RUNX1 and RPN1 contribute to chromosomal instability, myeloid leukemia proliferation, and a block in myeloid differentiation. Within aa 241-430, human EVI1 shares 94% aa sequence identity with mouse and rat EVI1.
Ecotropic Viral Integration Site 1
Entrez Gene IDs:
2122 (Human); 14013 (Mouse); 294924 (Rat)
AML1-EVI-1 fusion protein; AML1-EVI-1; ecotropic viral integration site 1; Ecotropic virus integration site 1 protein homolog; EVI1; EVI-1; EVI1MDS1-EVI1; MDS1 and EVI1 complex locus protein EVI1; MDS1 and EVI1 complex locus protein MDS1; MDS1 and EVI1 complex locus; MDS1; MECOM; MGC163392; MGC97004; Myelodysplasia syndrome 1 protein; myelodysplasia syndrome 1; Myelodysplasia syndrome-associated protein 1; oncogene EVI1; PRDM3; zinc finger protein Evi1
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