Human Fas Ligand/TNFSF6 Antibody

(9 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Fas Ligand/TNFSF6 in ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 100419
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Fas Ligand/TNFSF6
    Pro134-Leu281
    Accession # P48023
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
    • Human Fas Ligand/TNFSF6 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human Fas Ligand/TNFSF6 Antibody (Catalog # MAB126)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human Fas Ligand/TNFSF6 Biotinylated Antibody (Catalog # BAF126)
  • ELISA Standard
     
    Recombinant Human Fas Ligand/TNFSF6 Protein (Catalog # 126-FL)
  • Neutralization
    Measured by its ability to neutralize Fas Ligand/TNFSF6-induced cytotoxicity in the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND50) is typically 1-5 ng/mL in the presence of 2 ng/mL Recombinant Human Fas Ligand/TNFSF6 and 10 µg/mL of a cross-linking antibody, Mouse poly-Histidine Monoclonal Antibody.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cytotoxicity Induced by Fas Ligand/TNFSF6 and Neutralization by Human Fas Ligand/TNFSF6 Antibody. In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (Catalog # MAB050), Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL) induces cytotoxicity in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Under these conditions, cytotoxicity elicited by Recombinant Human Fas Ligand/TNFSF6 (2 ng/mL) is neutralized (green line) by increasing concentrations of Human Fas Ligand/TNFSF6 Monoclonal Antibody (Catalog # MAB126). The ND50 is typically 1-5 ng/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fas Ligand/TNFSF6
Fas Ligand (FasL), also known as CD178, CD95L, or TNFSF6, is a 40 kDa type II transmembrane member of the TNF superfamily of proteins. Its ability to induce apoptosis in target cells plays an important role in the development, homeostasis, and function of the immune system (1). Mature human Fas Ligand consists of a 179 amino acid (aa) extracellular domain (ECD), a 22 aa transmembrane segment, and a 80 aa cytoplasmic domain (2). Within the ECD, human Fas Ligand shares 81% and 78% aa sequence identity with mouse and rat Fas Ligand, respectively. Both mouse and human Fas Ligand are active on mouse and human cells (2, 3). Fas Ligand is expressed on the cell surface as a nondisulfide-linked homotrimer on activated CD4+ Th1 cells, CD8+ cytotoxic T cells, and NK cells (1). Fas Ligand binding to Fas/CD95 on an adjacent cell triggers apoptosis in the Fas‑expressing cell (2, 4). Fas Ligand also binds DcR3 which is a soluble decoy receptor that interferes with Fas Ligand-induced apoptosis (5). Fas Ligand can be released from the cell surface by metalloproteinases as a 26 kDa soluble molecule which remains trimeric (6, 7). Shed Fas Ligand retains the ability to bind Fas, although its ability to trigger apoptosis is dramatically reduced (6, 7). In the absence of TGF‑ beta, however, Fas Ligand/Fas interactions instead promote neutrophil-mediated inflammatory responses (3, 8). Fas Ligand itself transmits reverse signals that costimulate the proliferation of freshly antigen-stimulated T cells (9). Fas Ligand-induced apoptosis plays a central role in the development of immune tolerance and the maintance of immune privileged sites (10). This function is exploited by tumor cells which evade immune surveillance by upregulating Fas Ligand to kill tumor infiltrating lymphocytes (8, 11). In gld mice, a Fas Ligand point mutation is the cause of severe lymphoproliferation and systemic autoimmunity (12, 13).
  • References:
    1. Lettau, M. et al. (2008) Curr. Med. Chem. 15:1684.
    2. Takahashi, T. et al. (1994) Int. Immunol. 6:1567.
    3. Seino, K-I. et al. (1998) J. Immunol. 161:4484.
    4. Suda, T. et al. (1993) Cell 75:1169.
    5. Pitti, R.M. et al. (1998) Nature 396:699.
    6. Schneider, P. et al. (1998) J. Exp. Med. 187:1205.
    7. Tanaka, M. et al. (1998) Nature Med. 4:31.
    8. Chen, J-J. et al. (1998) Science 282:1714.
    9. Suzuki, I. and P.J. Fink (2000) Proc. Natl. Acad. Sci. USA 97:1707.
    10. Ferguson, T.A. and T.S. Griffith (2006) Immunol. Rev. 213:228.
    11. Ryan, A.E. et al. (2005) Cancer Res. 65:9817.
    12. Takahashi, T. et al. (1994) Cell 76:969.
    13. Lynch, D.H. et al. (1994) Immunity 1:131.
  • Entrez Gene IDs:
    356 (Human); 14103 (Mouse); 25385 (Rat)
  • Alternate Names:
    apoptosis (APO-1) antigen ligand 1; Apoptosis antigen ligand; APT1LG1CD95L; APTL; CD178 antigen; CD178; CD95L; CD95-L; Fas antigen ligand; Fas ligand (TNF superfamily, member 6); Fas Ligand; FASLCD95 ligand; FASLG; TNFSF6; TNFSF6FasL; tumor necrosis factor (ligand) superfamily, member 6; tumor necrosis factor ligand superfamily member 6
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Applications
Sample Type
  1. Biliary tract instillation of a SMAC mimetic induces TRAIL-dependent acute sclerosing cholangitis-like injury in mice
    Authors: ME Guicciardi, A Krishnan, SF Bronk, P Hirsova, TS Griffith, GJ Gores
    Cell Death Dis, 2017;8(1):e2535.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Combination of IAP antagonist and IFN? activates novel caspase-10- and RIPK1-dependent cell death pathways
    Authors: MC Tanzer, N Khan, JA Rickard, N Etemadi, N Lalaoui, SK Spall, JM Hildebrand, D Segal, M Miasari, D Chau, WL Wong, M McKinlay, SK Chunduru, CA Benetatos, SM Condon, JE Vince, MJ Herold, J Silke
    Cell Death Differ, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. Interleukin-10-producing LAG3(+) regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis
    Authors: S Nakachi, S Sumitomo, Y Tsuchida, H Tsuchiya, M Kono, R Kato, K Sakurai, N Hanata, Y Nagafuchi, S Tateishi, H Kanda, T Okamura, K Yamamoto, K Fujio
    Arthritis Res. Ther., 2017;19(1):97.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. The Therapeutic Effect of Pamidronate on Lethal Avian Influenza A H7N9 Virus Infected Humanized Mice.
    Authors: Zheng J, Wu W, Liu Y, Xiang Z, Liu M, Chan K, Lau S, Lam K, To K, Chan J, Li L, Chen H, Lau Y, Yuen K, Tu W
    PLoS ONE, 2015;10(8):e0135999.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. Reciprocal complementation of the tumoricidal effects of radiation and natural killer cells.
    Authors: Yang, Kai-Lin, Wang, Yu-Shan, Chang, Chao-Chu, Huang, Su-Chen, Huang, Yi-Chun, Chi, Mau-Shin, Chi, Kwan-Hwa
    PLoS ONE, 2013;8(4):e61797.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Potent anti-tumor killing activity of the multifunctional Treg cell line HOZOT against human tumors with diverse origins.
    Authors: Inoue T, Tashiro Y, Takeuchi M
    Int. J. Oncol., 2011;38(5):1299-306.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  7. KiSS1 metastasis suppressor gene product induces suppression of tyrosine kinase receptor signaling to Akt, tumor necrosis factor family ligand expression, and apoptosis.
    Authors: Navenot JM, Fujii N, Peiper SC
    Mol. Pharmacol., 2009;75(5):1074-83.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  8. Inhibition of p38 mitogen-activated protein kinase unmasks a CD30-triggered apoptotic pathway in anaplastic large cell lymphoma cells.
    Authors: Krysov SV, Krysov&lt;/LastName&gt;&lt;ForeNam SV&lt;/Initia, Rowley TF, Al-Shamkhani A
    Mol. Cancer Ther., 2007;6(2):703-11.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  9. A novel mechanism of CD40-induced apoptosis of carcinoma cells involving TRAF3 and JNK/AP-1 activation.
    Authors: Georgopoulos NT, Steele LP, Thomson MJ, Selby PJ, Southgate J, Trejdosiewicz LK
    Cell Death Differ., 2006;13(10):1789-801.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
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Mouse IgG2B Isotype Control

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Mouse IgG2B Isotype Control

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Mouse IgG2B Isotype Control

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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

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Mouse IgG HRP-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Mouse IgG2B Antibody

Flow MAB0043  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunohistochemistry.

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Images Ratings Applications Species Reviewed By Date Details
Immunohistochemistry Human Fas Ligand/TNFSF6 Antibody MAB126
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Very Good
 IHC Human Anonymous 11/04/2016
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Immunohistochemistry Human Fas Ligand/TNFSF6 Antibody MAB126
Immunohistochemistry: Human Fas Ligand/TNFSF6 Antibody [MAB126]

Summary

ApplicationImmunohistochemistry
Sample TestedAdult lung
SpeciesHuman

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