Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 962609
View all Ferritin Primary Antibodies »

Product Specifications

Immunogen

Human Ferritin synthetic peptide
Accession # P02794

Specificity

Detects human Ferritin in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Ferritin Antibody

Detection of Human Ferritin antibody by Western Blot.

Detection of Human Ferritin by Western Blot.

Western blot shows lysates of human liver tissue and human spleen tissue. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human Ferritin Monoclonal Antibody (Catalog # MAB93541) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Ferritin at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Ferritin antibody in iBJ6 iPS Cell Line by Immunocytochemistry (ICC).

Ferritin in iBJ6 iPS Cell Line.

Ferritin was detected in immersion fixed iBJ6 iPS cell line differentiated into hepatocytes using Mouse Anti-Human Ferritin Monoclonal Antibody (Catalog # MAB93541) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the perinuclear membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Ferritin Antibody

Application
Recommended Usage

Immunocytochemistry

1 µg/mL
Sample: Immersion fixed iBJ6 iPS cell line differentiated into hepatocytes

Western Blot

5 µg/mL
Sample: Human liver tissue and human spleen tissue

Reviewed Applications

Read 2 reviews rated 5 using MAB93541 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ferritin

FTH (Ferritin Heavy Chain 1; also Proliferation-Inducing Protein 15, PIG15, and PLIF) is a 183 aa cytosolic and secreted protein (SwissProt #: P02794).  Human FTH is highly conserved among species and shares 93% and 95% aa identity with mouse and rat FTH, respectively.  It is important for iron homeostatis by storing iron in a soluble form readily available to cells as well as having ferroxidase activity.  Additionally, FTH plays a role in iron delivery to cells and can be secreted in small amounts in the serum as an iron carrier.

Alternate Names

apoferritin, Cell proliferation-inducing gene 15 protein, Ferritin H subunit, ferritin heavy chain, ferritin, heavy polypeptide 1, FHC, FTHEC 1.16.3.1, FTHL6MGC104426, PIG15, placenta immunoregulatory factor, PLIF, proliferation-inducing protein 15

Entrez Gene IDs

2495 (Human)

Gene Symbol

FTH1

UniProt

P02794

Additional Ferritin Products

Product Documents for Human Ferritin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Ferritin Antibody

For research use only

Related Research Areas

Acute Phase Proteins

Citations for Human Ferritin Antibody

Customer Reviews for Human Ferritin Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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  • Human Ferritin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: hepatocytes
    Species: Human
    Verified Customer | Posted 06/07/2022
    Human Ferritin Antibody MAB93541
  • Human Ferritin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Spleen tissue
    Species: Human
    Verified Customer | Posted 02/22/2022
    Human Ferritin Antibody MAB93541

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Protocols

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