Human Ferritin Antibody

Catalog # Availability Size / Price Qty
MAB93541-100
MAB93541-SP
Detection of Human Ferritin by Western Blot.
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Human Ferritin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Ferritin in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2A Clone # 962609
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human Ferritin synthetic peptide
Accession # P02794
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
5 µg/mL
See below
Immunocytochemistry
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Ferritin antibody by Western Blot. View Larger

Detection of Human Ferritin by Western Blot. Western blot shows lysates of human liver tissue and human spleen tissue. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human Ferritin Monoclonal Antibody (Catalog # MAB93541) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Ferritin at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Ferritin antibody in iBJ6 iPS Cell Line by Immunocytochemistry (ICC). View Larger

Ferritin in iBJ6 iPS Cell Line. Ferritin was detected in immersion fixed iBJ6 iPS cell line differentiated into hepatocytes using Mouse Anti-Human Ferritin Monoclonal Antibody (Catalog # MAB93541) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the perinuclear membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ferritin

FTH (Ferritin Heavy Chain 1; also Proliferation-Inducing Protein 15, PIG15, and PLIF) is a 183 aa cytosolic and secreted protein (SwissProt #: P02794).  Human FTH is highly conserved among species and shares 93% and 95% aa identity with mouse and rat FTH, respectively.  It is important for iron homeostatis by storing iron in a soluble form readily available to cells as well as having ferroxidase activity.  Additionally, FTH plays a role in iron delivery to cells and can be secreted in small amounts in the serum as an iron carrier.

Entrez Gene IDs
2495 (Human)
Alternate Names
apoferritin; Cell proliferation-inducing gene 15 protein; Ferritin H subunit; ferritin heavy chain; Ferritin; ferritin, heavy polypeptide 1; FHC; FTHEC 1.16.3.1; FTHL6MGC104426; PIG15; placenta immunoregulatory factor; PLIF; proliferation-inducing protein 15

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Ferritin Antibody

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Human Ferritin Antibody
By Anonymous on 06/07/2022
Application: WB Sample Tested: hepatocytes Species: Human

Human Ferritin Antibody
By Anonymous on 02/22/2022
Application: WB Sample Tested: Spleen tissue Species: Human