Human FFAR2/GPR43 Antibody

Catalog # Availability Size / Price Qty
MAB10082-100
MAB10082-SP
Detection of FFAR2/GPR43 in HEK293 Human Cell Line Transfected with Human FFAR2/GPR43 and eGFP by Flow Cytometry.
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Human FFAR2/GPR43 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FFAR2/GPR43 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 1000624
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human FFAR2/GPR43
Met1-Glu330
Accession # O15552
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of FFAR2/GPR43 in HEK293 Human Cell Line Transfected with Human FFAR2/GPR43 and eGFP by Flow Cytometry. View Larger

Detection of FFAR2/GPR43 in HEK293 Human Cell Line Transfected with Human FFAR2/GPR43 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human FFAR2/GPR43 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human FFAR2/GPR43 Monoclonal Antibody (Catalog # MAB10082) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody(Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FFAR2/GPR43

Human Free fatty acid receptor 2 (FFA2, FFAR2, GPCR43 or GPR43) is a 330 aminoacids G-protein coupled receptor encoded by the FFAR2 gene. G-protein-coupled receptors (GPCRs) are seven-trans-membrane (7TM) receptors that mediate cellular responses to the majority of hormones and neurotransmitters and are common targets for drug discovery. Human GPR43/FFA2 is present in a large variety of tissues, including adipose tissue, inflammatory cells, and gastrointestinal (GI) tract and is activated by short-chain fatty acids (SCFAs). Studies on knockout mice have identified Free Fatty Acid Receptor 2 (FFAR2 or GPR43) as a critical gene in the prevention of obesity, colitis, asthma and arthritis.

Long Name
Free Fatty Acid Receptor 2
Entrez Gene IDs
2867 (Human); 233079 (Mouse); 292794 (Rat)
Alternate Names
FFAR2; free fatty acid activated receptor 2; free fatty acid receptor 2; G protein-coupled receptor 43; GPR43; GPR43FFA2R; G-protein coupled receptor 43

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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