Human FFAR2/GPR43 Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of FFAR2/GPR43 in HEK293 Human Cell Line Transfected with Human FFAR2/GPR43 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human FFAR2/GPR43 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human FFAR2/GPR43 Monoclonal Antibody (Catalog # MAB10082) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Background: FFAR2/GPR43

Human Free fatty acid receptor 2 (FFA2, FFAR2, GPCR43 or GPR43) is a 330 aminoacids G-protein coupled receptor encoded by the FFAR2 gene. G-protein-coupled receptors (GPCRs) are seven-trans-membrane (7TM) receptors that mediate cellular responses to the majority of hormones and neurotransmitters and are common targets for drug discovery. Human GPR43/FFA2 is present in a large variety of tissues, including adipose tissue, inflammatory cells, and gastrointestinal (GI) tract and is activated by short-chain fatty acids (SCFAs). Studies on knockout mice have identified Free Fatty Acid Receptor 2 (FFAR2 or GPR43) as a critical gene in the prevention of obesity, colitis, asthma and arthritis.

Product Datasheets

Related Research Areas

• Bioactive Lipid G Protein-Coupled Receptors
• Bioactive Lipids and Receptors
• G Protein-Coupled Receptors in Nutrition and Appetite
• G Protein-Coupled Receptors in Obesity and Related Metabolic Disorders
• Lipid and Fatty Acid Transport
• Nutrition and Appetite
• Obesity
• Steroid Hormones and Bioactive Lipids