Human Free fatty acid receptor 2 (FFA2, FFAR2, GPCR43 or GPR43) is a 330 aminoacids G-protein coupled receptor encoded by the FFAR2 gene. G-protein-coupled receptors (GPCRs) are seven-trans-membrane (7TM) receptors that mediate cellular responses to the majority of hormones and neurotransmitters and are common targets for drug discovery. Human GPR43/FFA2 is present in a large variety of tissues, including adipose tissue, inflammatory cells, and gastrointestinal (GI) tract and is activated by short-chain fatty acids (SCFAs). Studies on knockout mice have identified Free Fatty Acid Receptor 2 (FFAR2 or GPR43) as a critical gene in the prevention of obesity, colitis, asthma and arthritis.
Human FFAR2/GPR43 Antibody
R&D Systems | Catalog # MAB10082
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1000624
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Product Specifications
Immunogen
NS0 Mouse myeloma cell line transfected with human FFAR2/GPR43
Met1-Glu330
Accession # O15552
Met1-Glu330
Accession # O15552
Specificity
Stains human FFAR2/GPR43 transfectants but not irrelevant transfectants in flow cytometry.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human FFAR2/GPR43 Antibody
Detection of FFAR2/GPR43 in HEK293 Human Cell Line Transfected with Human FFAR2/GPR43 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with (A) human FFAR2/GPR43 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human FFAR2/GPR43 Monoclonal Antibody (Catalog # MAB10082) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.Applications for Human FFAR2/GPR43 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human FFAR2/GPR43 and eGFP
Sample: HEK293 Human Cell Line Transfected with Human FFAR2/GPR43 and eGFP
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: FFAR2/GPR43
Long Name
Free Fatty Acid Receptor 2
Alternate Names
GPR43
Gene Symbol
FFAR2
UniProt
Additional FFAR2/GPR43 Products
Product Documents for Human FFAR2/GPR43 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human FFAR2/GPR43 Antibody
For research use only
Related Research Areas
Citations for Human FFAR2/GPR43 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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