Detects human FGF-13 1B in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) FGF-3, -4, -5, -6, -7, -9, -10, -11, -12, -16, -17, -19, -20, -21, rhFGF basic, recombinant mouse FGF-8b, or -8c is observed.
Monoclonal Mouse IgG2A Clone # 298923
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human FGF-13 isoform 1B Ala2-Thr192 Accession # NP_378668
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Recombinant Human FGF‑13 1B
0.25 µg/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of FGF‑13 in RPMI 8226 Human Cell Line by Flow Cytometry. RPMI 8226 human multiple myeloma cell line was stained with Mouse Anti-Human FGF‑13 1B Monoclonal Antibody (Catalog # MAB1909, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
FGF-13 (Fibroblast Growth Factor-13), also known as FGF homologous factor 2 (FHF-2), is a member of the FGF family. It is expressed in brain and skeletal muscle and has been implicated in nervous system development and function. The gene for FGF-13 has been localized in human chromosome X, in a region associated with Borjeson-Forssman-Lehmann syndrome, a syndromal X-linked mental retardation. Two alternately spliced isoforms that differ in their amino-terminal residues have been described.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.