Detects human FGF-13 1B in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) FGF-3, -4, -5, -6, -7, -9, -10, -11, -12, -16, -17, -19, -20, -21, rhFGF basic, recombinant mouse FGF-8b, or -8c is observed.
Monoclonal Mouse IgG2A Clone # 298923
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human FGF-13 isoform 1B Ala2-Thr192 Accession # NP_378668
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of FGF‑13 in RPMI 8226 Human Cell Line by Flow Cytometry.
RPMI 8226 human multiple myeloma cell line was stained with Mouse Anti-Human FGF‑13 1B Monoclonal Antibody (Catalog # MAB1909, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
FGF-13 (Fibroblast Growth Factor-13), also known as FGF homologous factor 2 (FHF-2), is a member of the FGF family. It is expressed in brain and skeletal muscle and has been implicated in nervous system development and function. The gene for FGF-13 has been localized in human chromosome X, in a region associated with Borjeson-Forssman-Lehmann syndrome, a syndromal X-linked mental retardation. Two alternately spliced isoforms that differ in their amino-terminal residues have been described.
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