Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 133105
View all FGFR1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human FGF R1 alpha (IIIc)

Specificity

Detects human FGF R1 in direct ELISAs and Western blots. Recognizes all isoforms of recombinant human (rh) FGF R1 and shows

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human FGFR1 Antibody

Detection of Human FGFR1 by Western Blot

Detection of Human FGFR1 by Western Blot

Western blot confirmation of marker proteins. Equal quantities of protein from media supernatant from cells cultured as described in Fig 1 were run on Western blot and probed with antibodies for an E8-enriched marker protein (CHGA, NID1, SEMA3 or NPTX3) and an E6-enriched marker protein (COCH, FGFR1, Follistatin or OLFML3). Membranes were imaged using LICOR Odyssey system. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38875182), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human FGFR1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human FGF R1 alpha (IIIb) Fc Chimera (Catalog # 655-FR)
Recombinant Human FGF R1 alpha (IIIc) Fc Chimera (Catalog # 658-FR)
Recombinant Human FGF R1 beta (IIIb) Fc Chimera (Catalog # 765-FR)
Recombinant Human FGF R1 beta (IIIc) Fc Chimera (Catalog # 661-FR)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: FGFR1

FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGFR1 through 5. FGFRs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGFR1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGFR5 does not. Multiple forms of FGFR1, 2, and 3 are generated by alternative splicing.

Long Name

Fibroblast Growth Factor Receptor 1

Alternate Names

CD331, FGF R1, Flt-2

Entrez Gene IDs

2260 (Human); 14182 (Mouse)

Gene Symbol

FGFR1

Additional FGFR1 Products

Product Documents for Human FGFR1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human FGFR1 Antibody

For research use only

Citations for Human FGFR1 Antibody

Customer Reviews for Human FGFR1 Antibody

There are currently no reviews for this product. Be the first to review Human FGFR1 Antibody and earn rewards!

Have you used Human FGFR1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies