|Detection of Human FoxK1 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line. PVDF Membrane was probed with 1 µg/mL of Human FoxK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6366) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FoxK1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7.|
|FoxK1 in Human Colon. FoxK1 was detected in immersion fixed paraffin-embedded sections of human colon using Human FoxK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6366) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cell nuclei and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.|
FOX K1 (Forkhead box protein K1; also MNF) is a 75 kDa (predicted) nuclear protein that is a Class 1 member of the FOX family of transcription factors. It should not be confused with FOXK2/ILF1 that has also been referred to as FOXK1. It is widely expressed, but has become known as a marker for skeletal muscle satellite cells in the adult. Human FOXK1 is 733 amino acids (aa) in length. It contains one FHA domain that binds phosphopeptides (aa 116-203), plus a forkhead DNA binding region (aa 305-400) and over a dozen Ser/Thr phosphorylation sites. There are multiple potential splice variants. Two utilize an alternative start site at Met118 that may be accompanied by a 28 aa substitution for aa 673-733, while another contains 24 aa substitution for aa 1-187. Over aa 493-670, human FOXK1 shares 90% aa identity with mouse FOXK1.
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