Detects human Galectin-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows 100% cross-reactivity with recombinant mouse Galectin-3 and no cross-reactivity with rhGalectin-1, -2 -4, -7, or -8.
Monoclonal Mouse IgG2B Clone # 194804
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Galectin‑3 Ala2-Ile250 Accession # P17931
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human Galectin‑3 by Western Blot.
Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF‑7 human breast cancer cell line, and U‑118‑MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Galectin‑3 Monoclonal Antibody (Catalog # MAB1154) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Galectin‑3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Galectin-3, also known as Mac-2, L29, CBP35 and epsilon BP, is a member of a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells and fibroblasts.
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