Human Galectin-8 Antibody
Human Galectin-8 Antibody Summary
Met1-Trp317
Accession # O00214
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Galectin‑8 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Galectin-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Galectin-8 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Galectin‑8 in Human Lung. Galectin-8 was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human Galectin-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human Galectin‑8 by Simple WesternTM. Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin-8 at approximately 40 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Galectin-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Galectin-8
The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer. The chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain, and the tandem-repeat Galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1‑4).
Galectin-8, also known as prostate carcinoma tumor antigen 1 (PCTA1) in human, is a tandem repeat-type galectin. Prototype (single CRD) isoforms arising through alternate gene splicing have also been identified (5). Galectin-8 is highly expressed in lung carcinomas, certain forms of prostate carcinomas, as well as other tumor cells. It binds to a subset of cell surface integrins to modulate ECM-integrin interactions. As a soluble ligand, Galectin-8 can inhibit cell adhesion (6). Immobilized Galectin-8, however, has also been shown to promote cell adhesion (7). Human and mouse Galectin-8 share approximately 80% amino acid homology (4).
- Rabinovich, A. et al. (2002) TRENDS in Immunol. 23:313.
- Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
- Hughes, R.C. (2002) Biochimie 83:667.
- R&D Systems’ Cytokine Bulletin, Summer, 2002.
- Bidon, N. et al. (2001) Gene 274:253.
- Hadari, Y. et al. (1995) J. Biol. Chem. 270:3447.
- Levy, Y. et al. (2001) J. Biol. Chem. 276:31285.
Product Datasheets
Citations for Human Galectin-8 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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The C terminus of the mycobacterium ESX-1 secretion system substrate ESAT-6 is required for phagosomal membrane damage and virulence
Authors: MM Osman, JK Shanahan, F Chu, KK Takaki, ML Pinckert, AJ Pagán, R Brosch, WH Conrad, L Ramakrishn
Proceedings of the National Academy of Sciences of the United States of America, 2022;119(11):e2122161119.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Mechanistic insights into COVID-19 by global analysis of the SARS-CoV-2 3CLpro substrate degradome
Authors: I Pablos, Y Machado, HCR de Jesus, Y Mohamud, R Kappelhoff, C Lindskog, M Vlok, PA Bell, GS Butler, PM Grin, QT Cao, JP Nguyen, N Solis, S Abbina, W Rut, JC Vederas, L Szekely, A Szakos, M Drag, JN Kizhakkeda, K Mossman, JA Hirota, E Jan, H Luo, A Banerjee, CM Overall
Cell Reports, 2021;37(4):109892.
Species: Cercopithecus aethiops
Sample Types: Cell Lysates
Applications: Western Blot -
Ubiquitylation of lipopolysaccharide by RNF213 during bacterial infection
Authors: EG Otten, E Werner, A Crespillo-, KB Boyle, V Dharamdasa, C Pathe, B Santhanam, F Randow
Nature, 2021;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Mycobacterium marinum phthiocerol dimycocerosates enhance macrophage phagosomal permeabilization and membrane damage
Authors: MM Osman, AJ Pagán, JK Shanahan, L Ramakrishn
PLoS ONE, 2020;15(7):e0233252.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Structural insights into loss of function of a pore forming toxin and its role in pneumococcal adaptation to an intracellular lifestyle
Authors: DC Badgujar, A Anil, AE Green, MV Surve, S Madhavan, A Beckett, IA Prior, BK Godsora, SB Patil, PK More, SG Sarkar, A Mitchell, R Banerjee, PS Phale, TJ Mitchell, DR Neill, P Bhaumik, A Banerjee
PloS Pathogens, 2020;16(11):e1009016.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Human GBP1 Differentially Targets Salmonella and Toxoplasma to License Recognition of Microbial Ligands and Caspase-Mediated Death
Authors: D Fisch, B Clough, MC Domart, V Encheva, H Bando, AP Snijders, LM Collinson, M Yamamoto, AR Shenoy, EM Frickel
Cell Rep, 2020;32(6):108008.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Human trophoblast requires galectin-3 for cell migration and invasion
Authors: Ž Boji?-Trbo, M Jovanovi?, A Viloti?, N Kolundži?, I Stefanoska, F Zetterberg, UJ Nilsson, H Leffler, L Vi?ovac
Sci Rep, 2019;9(1):2136.
Species: Human
Sample Types: Basement Membrane Extract
Applications: ELISA Development (Detection) -
Phthiocerol dimycocerosates promote access to the cytosol and intracellular burden of Mycobacterium tuberculosis in lymphatic endothelial cells
Authors: TR Lerner, CJ Queval, A Fearns, U Repnik, G Griffiths, MG Gutierrez
BMC Biol., 2018;16(1):1.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
A Rab20-Dependent Membrane Trafficking Pathway Controls M.�tuberculosis Replication by Regulating Phagosome Spaciousness and Integrity
Authors: L Schnettger, A Rodgers, U Repnik, RP Lai, G Pei, M Verdoes, RJ Wilkinson, DB Young, MG Gutierrez
Cell Host Microbe, 2017;21(5):619-628.e5.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
K63-Linked Ubiquitination Targets Toxoplasma gondii for Endo-lysosomal Destruction in IFN?-Stimulated Human Cells
PLoS Pathog, 2016;12(11):e1006027.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases.
Authors: Meunier E, Dick M, Dreier R, Schurmann N, Kenzelmann Broz D, Warming S, Roose-Girma M, Bumann D, Kayagaki N, Takeda K, Yamamoto M, Broz P
Nature, 2014;509(7500):366-70.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Galectin-8 Promotes Cytoskeletal Rearrangement in Trabecular Meshwork Cells through Activation of Rho Signaling.
Authors: Diskin S, Chen WS, Cao Z, Gyawali S, Gong H, Soza A, Gonzalez A, Panjwani N
PLoS ONE, 2012;7(9):e44400.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Modulation of endothelial cell migration and angiogenesis: a novel function for the "tandem-repeat" lectin galectin-8.
Authors: Delgado VM, Nugnes LG, Colombo LL, Troncoso MF, Fernandez MM, Malchiodi EL, Frahm I, Croci DO, Compagno D, Rabinovich GA, Wolfenstein-Todel C, Elola MT
FASEB J., 2011;25(1):242-54.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Galectin-8 interacts with podoplanin and modulates lymphatic endothelial cell functions.
Authors: Cueni LN, Detmar M
Exp. Cell Res., 2009;315(10):1715-23.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
The galectin profile of the endothelium: altered expression and localization in activated and tumor endothelial cells.
Authors: Thijssen VL, Hulsmans S, Griffioen AW
Am. J. Pathol., 2008;172(2):545-53.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Galectin-8-mediated selective autophagy protects against seeded tau aggregation.
Authors: Falcon B, Noad J, McMahon H, Randow F, Goedert M
J Biol Chem, 0;293(7):2438-2451.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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Reviews for Human Galectin-8 Antibody
Average Rating: 4.6 (Based on 5 Reviews)
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The antibody can detect the higher oligomeric form of galectin-8 here. The 36 kDa version was not detectable due to increasing oligomerization of the protein.
Specificity: Specific
Sensitivity: Sensitive
Buffer: Loading buffer
Dilution: 1:1000
At 1:2000 dilution there was very specific binding and the detection was limited to crypts as observed here.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 1:1500 to 1:2000
This experiment, the cells were stained for galectin-8 antibody and then red color secondary was used to detect it.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 1:1000
The antibody is good for IFC also.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: PBS
Dilution: 1:1000
