Human Galectin-8 Antibody

(6 citations)
(4 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Galectin-8 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) rhGalectin-1 and rhGalectin-3 is observed, and less than 1% cross-reactivity with rhGalectin-2, rhGalectin-4 and rhGalectin-7 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Galectin-8
    Met1-Trp317
    Accession # O00214
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Galectin‑8 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Galectin‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Galectin‑8 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Galectin‑8 in Human Lung. Galectin‑8 was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human Galectin‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit  (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Galectin‑8 by Simple WesternTM. Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑8 at approximately 40 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Galectin‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Galectin-8

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer. The chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain, and the tandem-repeat Galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1‑4).

Galectin-8, also known as prostate carcinoma tumor antigen 1 (PCTA1) in human, is a tandem repeat-type galectin. Prototype (single CRD) isoforms arising through alternate gene splicing have also been identified (5). Galectin-8 is highly expressed in lung carcinomas, certain forms of prostate carcinomas, as well as other tumor cells. It binds to a subset of cell surface integrins to modulate ECM-integrin interactions. As a soluble ligand, Galectin-8 can inhibit cell adhesion (6). Immobilized Galectin-8, however, has also been shown to promote cell adhesion (7). Human and mouse Galectin-8 share approximately 80% amino acid homology (4).

  • References:
    1. Rabinovich, A. et al. (2002) TRENDS in Immunol. 23:313.
    2. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
    3. Hughes, R.C. (2002) Biochimie 83:667.
    4. R&D Systems’ Cytokine Bulletin, Summer, 2002.
    5. Bidon, N. et al. (2001) Gene 274:253.
    6. Hadari, Y. et al. (1995) J. Biol. Chem. 270:3447.
    7. Levy, Y. et al. (2001) J. Biol. Chem. 276:31285.
  • Entrez Gene IDs:
    3964 (Human)
  • Alternate Names:
    GAL8; Gal-8; galectin 8; Galectin8; Galectin-8; galectin-8g; lectin, galactoside-binding, soluble, 8; LGALS8; PCTA1; PCTA-1Po66 carbohydrate-binding protein; Po66 carbohydrate binding protein; Po66-CBP; Prostate carcinoma tumor antigen 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Applications
Sample Type
  1. K63-Linked Ubiquitination Targets Toxoplasma gondii for Endo-lysosomal Destruction in IFN?-Stimulated Human Cells
    PLoS Pathog, 2016;12(11):e1006027.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases.
    Authors: Meunier E, Dick M, Dreier R, Schurmann N, Kenzelmann Broz D, Warming S, Roose-Girma M, Bumann D, Kayagaki N, Takeda K, Yamamoto M, Broz P
    Nature, 2014;509(7500):366-70.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  3. Galectin-8 Promotes Cytoskeletal Rearrangement in Trabecular Meshwork Cells through Activation of Rho Signaling.
    Authors: Diskin S, Chen WS, Cao Z, Gyawali S, Gong H, Soza A, Gonzalez A, Panjwani N
    PLoS ONE, 2012;7(9):e44400.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Modulation of endothelial cell migration and angiogenesis: a novel function for the "tandem-repeat" lectin galectin-8.
    Authors: Delgado VM, Nugnes LG, Colombo LL, Troncoso MF, Fernandez MM, Malchiodi EL, Frahm I, Croci DO, Compagno D, Rabinovich GA, Wolfenstein-Todel C, Elola MT
    FASEB J., 2011;25(1):242-54.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Galectin-8 interacts with podoplanin and modulates lymphatic endothelial cell functions.
    Authors: Cueni LN, Detmar M
    Exp. Cell Res., 2009;315(10):1715-23.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  6. The galectin profile of the endothelium: altered expression and localization in activated and tumor endothelial cells.
    Authors: Thijssen VL, Hulsmans S, Griffioen AW
    Am. J. Pathol., 2008;172(2):545-53.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Cell and Tissue Staining Kits
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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
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Antigen Retrieval Reagent-Universal

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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

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Goat IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

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Goat IgG Horseradish Peroxidase-conjugated Antibody

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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Average Rating: 4.8 (Based on 4 reviews)

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We have 4 reviews tested in 1 species: Human.
We have 4 reviews tested in 3 applications: Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Human Galectin‑8 Antibody AF1305
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Very Good
 WB Human Anonymous 10/26/2015
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Image Details
Western Blot Human Galectin‑8 Antibody AF1305
Western Blot: Human Galectin‑8 Antibody [AF1305]

Summary

ApplicationWestern Blot
Sample TestedHuman recombinant galectin-8
SpeciesHuman

Other Experimental Details

Other Experimental DetailsThe antibody can detect the higher oligomeric form of galectin-8 here. The 36 kDa version was not detectable due to increasing oligomerization of the protein.
Specificity: Specific
Sensitivity: Sensitive
Buffer: Loading buffer
Dilution: 1:1000
Immunohistochemistry Human Galectin‑8 Antibody AF1305
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Excellent
 IHC Human Anonymous 10/26/2015
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Immunohistochemistry Human Galectin‑8 Antibody AF1305
Immunohistochemistry: Human Galectin‑8 Antibody [AF1305]

Summary

ApplicationImmunohistochemistry
Sample TestedColon tissue
SpeciesHuman

Other Experimental Details

Other Experimental DetailsAt 1:2000 dilution there was very specific binding and the detection was limited to crypts as observed here.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 1:1500 to 1:2000
Immunocytochemistry/Immunofluorescence Human Galectin‑8 Antibody AF1305
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Excellent
 ICC/IF Human Anonymous 10/26/2015
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Immunocytochemistry/Immunofluorescence Human Galectin‑8 Antibody AF1305
Immunocytochemistry/Immunofluorescence: Human Galectin‑8 Antibody [AF1305]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedHuman cells
SpeciesHuman

Other Experimental Details

Other Experimental DetailsThis experiment, the cells were stained for galectin-8 antibody and then red color secondary was used to detect it.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 1:1000
Immunocytochemistry/Immunofluorescence Human Galectin‑8 Antibody AF1305
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Excellent
 ICC/IF Human Anonymous 10/26/2015
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Immunocytochemistry/Immunofluorescence Human Galectin‑8 Antibody AF1305
Immunocytochemistry/Immunofluorescence: Human Galectin‑8 Antibody [AF1305]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedCancer Cells
SpeciesHuman

Other Experimental Details

Other Experimental DetailsThe antibody is good for IFC also.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: PBS
Dilution: 1:1000

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