GAPDH-2 (Glyceraldehyde-3-phosphate dehydrogenase 2; also GAPD2 and GAPDS) is a 40-44 kDa member of the GAPDH family of enzymes. Due to a high incidence of proline residues, it may migrate anomalously at 56 kDa in SDS-PAGE. GAPDH-2 is expressed only in testis as a homotetramer, where it is found in the cytoplasm bound to the sperm fibrous sheath. Unlike GAPDH that has multiple functions, GAPDH-2 appears to participate only in carbohydrate metabolism, generating energy via glycolysis for sperm motility and fertility. Human GAPDH-2 is 408 amino acids (aa) in length. There are two NAD binding sites (Asp106 and Asn388) with a catalytic region between aa 223-226. One splice variant shows a nine aa insertion after Ser220. Over aa 75-222, human GAPDH-2 shares 84% aa identity with mouse GAPDH-2.
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human GAPDH-2
Glu75-Ala222
Accession # O14556
Glu75-Ala222
Accession # O14556
Specificity
Detects human GAPDH-2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human GAPDH and recombinant mouse GAPDH is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human GAPDH-2 Antibody
Detection of Human GAPDH‑2 by Western Blot.
Western blot shows lysates of human testis tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human GAPDH-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6276) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GAPDH-2 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GAPDH‑2 in Human Testes.
GAPDH-2 was detected in immersion fixed paraffin-embedded sections of human testes using Goat Anti-Human GAPDH-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6276) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of sperm cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human GAPDH-2 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human testes
Sample: Immersion fixed paraffin-embedded sections of human testes
Western Blot
1 µg/mL
Sample: Human testis tissue
Sample: Human testis tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: GAPDH-2
Long Name
Glyceraldehyde-3-phosphate Dehydrogenase 2
Alternate Names
GAPD2, GAPDH2, GAPDHS, HSD-35
Gene Symbol
GAPDHS
UniProt
Additional GAPDH-2 Products
Product Documents for Human GAPDH-2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human GAPDH-2 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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