Human GCN2 Antibody

Catalog # Availability Size / Price Qty
Detection of Human GCN2 by Western Blot.
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Product Details
Supplemental Products

Human GCN2 Antibody Summary

Species Reactivity
Detects human GCN2 in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 708210
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human GCN2
Accession # Q9P2K8
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human GCN2 antibody by Western Blot. View Larger

Detection of Human GCN2 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GCN2 Monoclonal Antibody (Catalog # MAB6878) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GCN2 at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GCN2

GCN2, also known as EIF2AK4, is a widely expressed 190 kDa ribosome-associated Ser/Thr kinase that plays an important role in the control of protein translation. In response to amino acid deprivation or UV irradiation, GCN2 phosphorylates the alpha subunit of eukaryotic initiation factor 2 (eIF2a) at Ser51 and induces a delay in entry to S phase of the cell cycle. Increased GCN2 activity in solid tumors enhances the production of amino acids and contributes to tumor cell survival in conditions of nutrient deprivation. GCN2 contains an RWD domain (aa 21-134), a pseudo-kinase domain (aa 296-539), a catalytic domain (aa 590-1001), and a histidyl tRNA synthase-like domain (aa 1022-1493) followed by a C-terminal ribosome interacting region. Alternate splicing generates an isoform with a short deletion in the kinase domain and an isoform that is truncated at the N-terminal end of the kinase domain. Within aa 22-139, human GCN2 shares 91% aa sequence identity with mouse and rat GCN2.

Long Name
General Control Nonrepressed 2-like Protein
Entrez Gene IDs
440275 (Human); 27103 (Mouse); 114859 (Rat)
Alternate Names
EIF2AK4; eukaryotic translation initiation factor 2 alpha kinase 4; eukaryotic translation initiation factor 2-alpha kinase 4; GCN2; GCN2GCN2 eIF2alpha kinase; GCN2-like protein; KIAA1338EC

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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