Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 708210
View all GCN2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GCN2
Arg22-Lys139
Accession # Q9P2K8

Specificity

Detects human GCN2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human GCN2 Antibody

Detection of Human GCN2 antibody by Western Blot.

Detection of Human GCN2 by Western Blot.

Western blot shows lysates of 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GCN2 Monoclonal Antibody (Catalog # MAB6878) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GCN2 at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human GCN2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GCN2

GCN2, also known as EIF2AK4, is a widely expressed 190 kDa ribosome-associated Ser/Thr kinase that plays an important role in the control of protein translation. In response to amino acid deprivation or UV irradiation, GCN2 phosphorylates the alpha subunit of eukaryotic initiation factor 2 (eIF2a) at Ser51 and induces a delay in entry to S phase of the cell cycle. Increased GCN2 activity in solid tumors enhances the production of amino acids and contributes to tumor cell survival in conditions of nutrient deprivation. GCN2 contains an RWD domain (aa 21-134), a pseudo-kinase domain (aa 296-539), a catalytic domain (aa 590-1001), and a histidyl tRNA synthase-like domain (aa 1022-1493) followed by a C-terminal ribosome interacting region. Alternate splicing generates an isoform with a short deletion in the kinase domain and an isoform that is truncated at the N-terminal end of the kinase domain. Within aa 22-139, human GCN2 shares 91% aa sequence identity with mouse and rat GCN2.

Long Name

General Control Nonrepressed 2-like Protein

Alternate Names

EIF2AK4

Entrez Gene IDs

440275 (Human); 27103 (Mouse); 114859 (Rat)

Gene Symbol

EIF2AK4

UniProt

Q9P2K8

Additional GCN2 Products

Product Documents for Human GCN2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GCN2 Antibody

For research use only

Citations for Human GCN2 Antibody

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