Human GFAP Antibody

(6 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human GFAP in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 273807
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human GFAP
    Leu292-Met432
    Accession # P14136
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    50 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human GFAP by Western Blot. Western blot shows lysates of human brain (motor cortex) tissue, human brain (cerebellum) tissue, and human brain (hypothalamus) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB2594) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for GFAP at approximately 35-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
GFAP in Rat Cortical Stem Cells. GFAP was detected in immersion fixed differentiated rat cortical stem cells using Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB2594) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Detection of Human GFAP by Simple WesternTM. Simple Western lane view shows lysates of human brain (cerebellum) tissue and human brain (motor cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for GFAP at approximately 51-52 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB2594). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GFAP
Glial Fibrillary Acidic Protein (GFAP) is the predominant component of astrocyte intermediate filaments in the central nervous system. It has also been detected in the glial cells of the enteric nervous system and some Schwann cells in the peripheral nervous systems.
  • Long Name:
    Glial Fibrillary Acidic Protein
  • Entrez Gene IDs:
    2670 (Human); 14580 (Mouse); 24387 (Rat)
  • Alternate Names:
    FLJ45472; GFAP astrocytes; GFAP; glial fibrillary acidic protein
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. MicroRNA-210 overexpression induces angiogenesis and neurogenesis in the normal adult mouse brain.
    Authors: Zeng, L, He, X, Wang, Y, Tang, Y, Zheng, C, Cai, H, Liu, J, Wang, Y, Fu, Y, Yang, G-Y
    Gene Ther, 2014;21(1):37-43.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-OCT embedded
  2. Enhanced sphingosine-1-phosphate receptor 2 expression underlies female CNS autoimmunity susceptibility.
    Authors: Cruz-Orengo L, Daniels B, Dorsey D, Basak S, Grajales-Reyes J, McCandless E, Piccio L, Schmidt R, Cross A, Crosby S, Klein R
    J Clin Invest, 2014;124(6):2571-84.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  3. Gene expression profile of glioblastoma peritumoral tissue: an ex vivo study.
    Authors: Mangiola, Annunzia, Saulnier, Nathalie, De Bonis, Pasquale, Orteschi, Daniela, Sica, Gigliola, Lama, Gina, Pettorini, Benedett, Sabatino, Giovanni, Zollino, Marcella, Lauriola, Libero, Colabianchi, Anna, Proietti, Gabriell, Kovacs, Gyula, Maira, Giulio, Anile, Carmelo
    PLoS ONE, 2013;8(3):e57145.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  4. CD133 positive embryonal rhabdomyosarcoma stem-like cell population is enriched in rhabdospheres.
    Authors: Walter D, Satheesha S, Albrecht P, Bornhauser BC, D'Alessandro V, Oesch SM, Rehrauer H, Leuschner I, Koscielniak E, Gengler C, Moch H, Bernasconi M, Niggli FK, Schafer BW
    PLoS ONE, 2011;6(5):e19506.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  5. Presence of pluripotent CD133+ cells correlates with malignancy of gliomas.
    Authors: Thon N, Damianoff K, Hegermann J, Grau S, Krebs B, Schnell O, Tonn JC, Goldbrunner R
    Mol. Cell. Neurosci., 2010;43(1):51-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  6. Development of a culture system that supports adult microglial cell proliferation and maintenance in the resting state.
    Authors: Ponomarev ED, Novikova M, Maresz K, Shriver LP, Dittel BN
    J. Immunol. Methods, 2005;300(1):32-46.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
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