Human Glucagon R/GCGR Antibody New

MAB10296 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10296-100
MAB10296-SP

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Human Glucagon R/GCGR Antibody in Flow Cytometry

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Glucagon R/GCGR Antibody Summary

Species Reactivity

Human

Specificity

Detects human Glucagon R/GCGR in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 591929

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with human Glucagon R/GCGR
Met1-Gln142
Accession # P47871

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry

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Detection of Glucagon R/GCGR in HEK293 Human Cell Line Transfected with Human Glucagon R/GCGR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human Glucagon R/GCGR or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Glucagon R/GCGR Monoclonal Antibody (Catalog # MAB10296) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry

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Glucagon R/GCGR in Human Pancreas. Glucagon R/GCGR was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Glucagon R/GCGR Monoclonal Antibody (Catalog # MAB10296) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glucagon R/GCGR

GLR (GCGR/Glucagon Receptor) is a GPCR for glucagon that plays a role in blood glucose levels and glucose homeostasis. Glucagon receptors are mainly expressed in liver and kidney but also expressed in other tissues such as heart, adipose, spleen, thymus, adrenal glands, pancreas, cerebral cortex and the gastrointestinal tract. Mutations in the GCGR gene is associated with Type 2 Diabetes Mellitus and inactivation of the receptor causes glucagon resistance. GCGR is found on the cell membrane but is rapidly internalized following ligand-binding via endocytosis.

Long Name

Glucagon Receptor

Entrez Gene IDs