Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 591929
View all Glucagon R/GCGR Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human Glucagon R/GCGR
Met1-Gln142
Accession # P47871

Specificity

Detects human Glucagon R/GCGR in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Glucagon R/GCGR Antibody

Detection of Glucagon R/GCGR antibody in HEK293 Human Cell Line Transfected with Human Glucagon R/GCGR and eGFP antibody by Flow Cytometry.

Detection of Glucagon R/GCGR in HEK293 Human Cell Line Transfected with Human Glucagon R/GCGR and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) human Glucagon R/GCGR or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Glucagon R/GCGR Monoclonal Antibody (Catalog # MAB10296) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Glucagon R/GCGR Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human Glucagon R/GCGR and eGFP

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glucagon R/GCGR

GLR (GCGR/Glucagon Receptor) is a GPCR for glucagon that plays a role in blood glucose levels and glucose homeostasis. Glucagon receptors are mainly expressed in liver and kidney but also expressed in other tissues such as heart, adipose, spleen, thymus, adrenal glands, pancreas, cerebral cortex and the gastrointestinal tract. Mutations in the GCGR gene is associated with Type 2 Diabetes Mellitus and inactivation of the receptor causes glucagon resistance. GCGR is found on the cell membrane but is rapidly internalized following ligand-binding via endocytosis.

Long Name

Glucagon Receptor

Alternate Names

GCGR, GGR, GLR, GlucagonR

Entrez Gene IDs

2642 (Human); 14527 (Mouse)

Gene Symbol

GCGR

UniProt

P47871

Additional Glucagon R/GCGR Products

Product Documents for Human Glucagon R/GCGR Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Glucagon R/GCGR Antibody

For research use only

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