Human Glucagon R/GCGR Antibody Summary
Accession # P47871
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Glucagon R/GCGR in HEK293 Human Cell Line Transfected with Human Glucagon R/GCGR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human Glucagon R/GCGR or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Glucagon R/GCGR Monoclonal Antibody (Catalog # MAB10296) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.
Glucagon R/GCGR in Human Pancreas. Glucagon R/GCGR was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Glucagon R/GCGR Monoclonal Antibody (Catalog # MAB10296) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glucagon R/GCGR
GLR (GCGR/Glucagon Receptor) is a GPCR for glucagon that plays a role in blood glucose levels and glucose homeostasis. Glucagon receptors are mainly expressed in liver and kidney but also expressed in other tissues such as heart, adipose, spleen, thymus, adrenal glands, pancreas, cerebral cortex and the gastrointestinal tract. Mutations in the GCGR gene is associated with Type 2 Diabetes Mellitus and inactivation of the receptor causes glucagon resistance. GCGR is found on the cell membrane but is rapidly internalized following ligand-binding via endocytosis.
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Cell and Tissue Staining Kits
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