Human Glycoprotein V/CD42d Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Glycoprotein V/CD42d in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Glycoprotein VI, rhTLR1, or rhTLR2 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 508219
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Glycoprotein V/CD42d
    Gln17-Gly523 (predicted)
    Accession # P40197
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Glycoprotein V/CD42d by Western Blot. Western blot shows lysates of human platelets. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Glycoprotein V/
CD42d Monoclonal Antibody (Catalog # MAB42491) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glycoprotein V/CD42d at approximately 83 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glycoprotein V/CD42d

GPV (platelet glycoprotein V; designated CD42d) is an 83 kDa type I transmembrane (TM) glycoprotein of the leucine‑rich repeat (LRR) family (1, 2). It is expressed exclusively within the platelet / megakaryocyte lineage, where it noncovalently interacts with other platelet TM LRR proteins, GPIb alpha / beta and GPIX, at a ratio of one GPV to two of each other subunit (2). The GPI‑V‑IX complex tethers platelets to von Willebrand factor on the surface of injured endothelial cells. Absence of the complex results in Bernard‑Soulier syndrome, a rare bleeding disorder (1‑3). The human GPV cDNA encodes a 560 amino acid (aa) protein with a 16 aa signal sequence, a 507 aa extracellular domain (ECD) containing 15 LRR, a 21 aa TM sequence, and a short (16 aa) cytoplasmic tail that binds calmodulin in resting, but not activated platelets. The human GPV ECD shares 70%, 71% and 81% aa identity with mouse, rat and equine GPV, respectively. GPV can form soluble fragments of 80 kDa by ADAM10 or ADAM17 cleavage after P507, or 69 kDa by thrombin cleavage after R476 (1, 4, 5). High circulating soluble GPV may be an indicator of platelet activation, but may also be caused by high doses of aspirin (6‑8). The function of GPV is not entirely clear. Deletion of GPV in mice does not produce any obvious change to surface expression or function of GPIb and GPIX, but surface expression of GPV requires GPIb (9, 10). Deletion studies also indicate that GPV may play a minor role in collagen adhesion, and may modify platelet aggregation in response to thrombin (3, 11‑15).

  • References:
    1. Lanza, F. et al. (1993) J. Biol. Chem. 268:20801.
    2. Hickey, M.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:8327.
    3. Ozaki, Y. et al. (2005) J. Thromb. Haemost. 3:1745.
    4. Rabie, T. et al. (2005) J. Biol. Chem. 280:14462.
    5. Gardiner, E.E. et al. (2007) J. Thromb. Haemost. 5:1530.
    6. Wolff, V. et al. (2005) Stroke 36:e17.
    7. Javela, K. et al. (2005) Transfusion 45:1504.
    8. Aktas, B. et al. (2005) J. Biol. Chem. 280:39716.
    9. Kahn, M.L. (1999) Blood 94:4112.
    10. Strassel, C. et al. (2004) Eur. J. Biochem. 271:3671.
    11. Nonne, C. et al. (2008) J. Thromb. Haemost. 6:210.
    12. Moog, S. et al. (2001) Blood 98:1038.
    13. Ramakrishnan, V. et al. (1999) Proc. Natl. Acad. Sci. USA 96:13336.
    14. Ni, H. et al. (2001) Blood 98:368.
    15. Andrews, R.K. et al. (2001) Blood 98:681.
  • Entrez Gene IDs:
    2814 (Human); 14729 (Mouse); 25259 (Rat)
  • Alternate Names:
    platelet glycoprotein V; CD42d; glycoprotein V (platelet); Glycoprotein V; GP5; GPV
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

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Goat Anti-Mouse IgG Biotinylated Antibody

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Mouse IgG Antibody

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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Mouse IgG VisUCyte HRP Polymer Antibody

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Rat Anti-Mouse IgG2B APC-conjugated Antibody

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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Mouse IgG2B Antibody

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Rat Anti-Mouse IgG2B PE-conjugated Antibody

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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

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