Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 508219
View all Glycoprotein V/CD42d Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Glycoprotein V/CD42d
Gln17-Gly523 (predicted)
Accession # P40197

Specificity

Detects human Glycoprotein V/CD42d in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Glycoprotein VI, rhTLR1, or rhTLR2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Glycoprotein V/CD42d Antibody

Detection of Human Glycoprotein V/CD42d antibody by Western Blot.

Detection of Human Glycoprotein V/CD42d by Western Blot.

Western blot shows lysates of human platelets. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Glycoprotein V/ CD42d Monoclonal Antibody (Catalog # MAB42491) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glycoprotein V/CD42d at approximately 83 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Glycoprotein V/CD42d Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human platelet

Reviewed Applications

Read 2 reviews rated 3.5 using MAB42491 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glycoprotein V/CD42d

GPV (platelet glycoprotein V; designated CD42d) is an 83 kDa type I transmembrane (TM) glycoprotein of the leucine‑rich repeat (LRR) family (1, 2). It is expressed exclusively within the platelet / megakaryocyte lineage, where it noncovalently interacts with other platelet TM LRR proteins, GPIb alpha / beta and GPIX, at a ratio of one GPV to two of each other subunit (2). The GPI‑V‑IX complex tethers platelets to von Willebrand factor on the surface of injured endothelial cells. Absence of the complex results in Bernard‑Soulier syndrome, a rare bleeding disorder (1‑3). The human GPV cDNA encodes a 560 amino acid (aa) protein with a 16 aa signal sequence, a 507 aa extracellular domain (ECD) containing 15 LRR, a 21 aa TM sequence, and a short (16 aa) cytoplasmic tail that binds calmodulin in resting, but not activated platelets. The human GPV ECD shares 70%, 71% and 81% aa identity with mouse, rat and equine GPV, respectively. GPV can form soluble fragments of 80 kDa by ADAM10 or ADAM17 cleavage after P507, or 69 kDa by thrombin cleavage after R476 (1, 4, 5). High circulating soluble GPV may be an indicator of platelet activation, but may also be caused by high doses of aspirin (6‑8). The function of GPV is not entirely clear. Deletion of GPV in mice does not produce any obvious change to surface expression or function of GPIb and GPIX, but surface expression of GPV requires GPIb (9, 10). Deletion studies also indicate that GPV may play a minor role in collagen adhesion, and may modify platelet aggregation in response to thrombin (3, 11‑15).

References

  1. Lanza, F. et al. (1993) J. Biol. Chem. 268:20801.
  2. Hickey, M.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:8327.
  3. Ozaki, Y. et al. (2005) J. Thromb. Haemost. 3:1745.
  4. Rabie, T. et al. (2005) J. Biol. Chem. 280:14462.
  5. Gardiner, E.E. et al. (2007) J. Thromb. Haemost. 5:1530.
  6. Wolff, V. et al. (2005) Stroke 36:e17.
  7. Javela, K. et al. (2005) Transfusion 45:1504.
  8. Aktas, B. et al. (2005) J. Biol. Chem. 280:39716.
  9. Kahn, M.L. (1999) Blood 94:4112.
  10. Strassel, C. et al. (2004) Eur. J. Biochem. 271:3671.
  11. Nonne, C. et al. (2008) J. Thromb. Haemost. 6:210.
  12. Moog, S. et al. (2001) Blood 98:1038.
  13. Ramakrishnan, V. et al. (1999) Proc. Natl. Acad. Sci. USA 96:13336.
  14. Ni, H. et al. (2001) Blood 98:368.
  15. Andrews, R.K. et al. (2001) Blood 98:681.

Alternate Names

CD42d, GP5, GPV

Entrez Gene IDs

2814 (Human); 14729 (Mouse); 25259 (Rat)

Gene Symbol

GP5

UniProt

P40197 (Human)

Additional Glycoprotein V/CD42d Products

Product Documents for Human Glycoprotein V/CD42d Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Glycoprotein V/CD42d Antibody

For research use only

Related Research Areas

Coagulation
Myeloid Lineage Markers

Customer Reviews for Human Glycoprotein V/CD42d Antibody (2)

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2 Customer Ratings
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  • Human Glycoprotein V/CD42d Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/03/2020
    Human Glycoprotein V/CD42d Antibody MAB42491
  • Human Glycoprotein V/CD42d Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/09/2018

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