G Protein-coupled Receptor 39 (GPR39) is a seven-transmembrane glycoprotein and a member of the Ghrelin receptor family. GPR39 is expressed in the stomach, small intestine and areas of the brain including the hypothalamus; its ligand is the Ghrelin-associated peptide, Obestatin. Obestatin decelerates gastric emptying and decreases intestinal contractility in mice, counteracting the effects of Ghrelin. GPR39 has not been shown to be internalized upon ligand binding. Extracellular loops of human GPR39 show 80% amino acid identity with mouse GPR39.
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 189702
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Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human GPR39
Met1-Val453
Accession # BAG37409
Met1-Val453
Accession # BAG37409
Specificity
Detects human GPR39. Stains human GPR39-transfected cells but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human GPR39 Antibody
Detection of GPR39 in NS0 Mouse Cell Line Transfected with Human GPR39 by Flow Cytometry.
NS0 mouse myeloma cell line transfected with human GPR39 was stained with Mouse Anti-Human GPR39 Monoclonal Antibody (Catalog # MAB3348, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).Applications for Human GPR39 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: NS0 mouse myeloma cell line transfected with human GPR39
Sample: NS0 mouse myeloma cell line transfected with human GPR39
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: GPR39
Long Name
G Protein-coupled Receptor 39
Alternate Names
G protein-coupled receptor 39, G-protein coupled receptor 39, MGC149541
Gene Symbol
GPR39
UniProt
Additional GPR39 Products
Product Documents for Human GPR39 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human GPR39 Antibody
For research use only
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