Human Granzyme K Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB10216-100
MAB10216-SP
Detection  of Human Granzyme K by Western Blot.
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Human Granzyme K Antibody Summary

Species Reactivity
Human
Specificity
Detects human Granzyme K in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2471A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Synthetic peptide containing human Granzyme K
Accession # P49863
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunohistochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human Granzyme K by Western Blot. View Larger

Detection of Human Granzyme K by Western Blot. Western blot shows lysates of human NK cells, U2OS human osteosarcoma cell line, and RT-4 human bladder carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Granzyme K Monoclonal Antibody (Catalog # MAB10216) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Granzyme K at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Granzyme  K in Human Tonsil. View Larger

Granzyme K in Human Tonsil. Granzyme K was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human Granzyme K Monoclonal Antibody (Catalog # MAB10216) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Granzyme K

Granzymes are released by cytoplasmic granules within NK and cytotoxic T cells.  They are serine proteases that induce apoptosis in the target cell.  Granzymes have also been found to help initiate the inflammatory response by activating macrophages and mast cells when in an extracellular state.  Granzymes have also been found to protect the body against the formation of different kinds of lymphomas.

References
  1. Bots, M. and JP Medema (2006). J.Cell Sci. 119:5011.
  2. Walch, M. et al. (2014). Cell. 157:1309.
  3. Cullen, SP. et al. (2010). Cell Death Differ. 17:616.
Entrez Gene IDs
3003 (Human); 14945 (Mouse); 29165 (Rat)
Alternate Names
EC 3.4.21; EC 3.4.21.-; EC 3.4.21.4; Fragmentin-3; Granzyme 3; granzyme K (granzyme 3; tryptase II); granzyme K (serine protease, granzyme 3; tryptase II); Granzyme K; Granzyme-3; Granzyme-K; GZMK; NK-Tryp-2; NK-Tryptase-2; PRSS; TRYP2; TRYP2granzyme-3; Tryptase II

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Reconstitution Buffers

Secondary Antibodies

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