Human Granzyme K Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty

Save Up to 40% on RUO Reagents with BIOSPRING24 (See Details)

Detection of Human Granzyme K by Western Blot.
3 Images
Product Details
Supplemental Products

Human Granzyme K Antibody Summary

Species Reactivity
Detects human Granzyme K in direct ELISAs.
Recombinant Monoclonal Rabbit IgG Clone # 2471A
Protein A or G purified from cell culture supernatant
Synthetic peptide containing human Granzyme K
Accession # P49863
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
See below
Flow Cytometry
25 µg/mL
Human peripheral blood mononuclear cells (PBMCs) 
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Granzyme K antibody by Western Blot. View Larger

Detection of Human Granzyme K by Western Blot. Western blot shows lysates of human NK cells, U2OS human osteosarcoma cell line, and RT-4 human bladder carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Granzyme K Monoclonal Antibody (Catalog # MAB10216) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Granzyme K at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Granzyme K antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

Granzyme K in Human Tonsil. Granzyme K was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human Granzyme K Monoclonal Antibody (Catalog # MAB10216) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Flow Cytometry View Larger

Detection of Granzyme K in expanded Human NK cells by Flow Cytometry. Detection of Granzyme K in expanded Human NK cells by Flow Cytometry. NK cells were expanded from Human peripheral blood mononuclear cells (PBMCs) for 14 days using ExCellerate™ Human NK Cell Expansion Media (CCM032). NK cells were stained with (A) Mouse Anti-Human Granzyme K Monoclonal Antibody (Catalog # MAB10216), followed by PE-conjugated anti-Rabbit IgG secondary antibody (F0110) and Rabbit Anti-Human CD56 Alexa Fluor® 647-conjugated Monoclonal Antibody (FAB24086R). (B) Quadrant markers were set based on control antibody staining (MAB1050). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Granzyme K

Granzymes are released by cytoplasmic granules within NK and cytotoxic T cells.  They are serine proteases that induce apoptosis in the target cell.  Granzymes have also been found to help initiate the inflammatory response by activating macrophages and mast cells when in an extracellular state.  Granzymes have also been found to protect the body against the formation of different kinds of lymphomas.

  1. Bots, M. and JP Medema (2006). J.Cell Sci. 119:5011.
  2. Walch, M. et al. (2014). Cell. 157:1309.
  3. Cullen, SP. et al. (2010). Cell Death Differ. 17:616.
Entrez Gene IDs
3003 (Human); 14945 (Mouse); 29165 (Rat)
Alternate Names
EC 3.4.21; EC 3.4.21.-; EC; Fragmentin-3; Granzyme 3; granzyme K (granzyme 3; tryptase II); granzyme K (serine protease, granzyme 3; tryptase II); Granzyme K; Granzyme-3; Granzyme-K; GZMK; NK-Tryp-2; NK-Tryptase-2; PRSS; TRYP2; TRYP2granzyme-3; Tryptase II

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Granzyme K Antibody

There are currently no reviews for this product. Be the first to review Human Granzyme K Antibody and earn rewards!

Have you used Human Granzyme K Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review