Human Granzyme K Antibody Summary
Accession # P49863
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Granzyme K by Western Blot. Western blot shows lysates of human NK cells, U2OS human osteosarcoma cell line, and RT-4 human bladder carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Granzyme K Monoclonal Antibody (Catalog # MAB10216) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Granzyme K at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Granzyme K in Human Tonsil. Granzyme K was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human Granzyme K Monoclonal Antibody (Catalog # MAB10216) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Granzyme K
Granzymes are released by cytoplasmic granules within NK and cytotoxic T cells. They are serine proteases that induce apoptosis in the target cell. Granzymes have also been found to help initiate the inflammatory response by activating macrophages and mast cells when in an extracellular state. Granzymes have also been found to protect the body against the formation of different kinds of lymphomas.
- Bots, M. and JP Medema (2006). J.Cell Sci. 119:5011.
- Walch, M. et al. (2014). Cell. 157:1309.
- Cullen, SP. et al. (2010). Cell Death Differ. 17:616.
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