Human Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2A Clone # 584308
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1
    Pro38-Trp411
    Accession # O60243
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    Immersion fixed paraffin-embedded sections of human liver and human liver cancer tissue
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1

Heparan Sulfate is a highly sulfated polysaccharide that can be found on cell surface and extracellular matrix. It is usually covalently attached to a protein core as the glycan component of a proteoglycan. Heparan Sulfate interacts with a variety of proteins, such as growth factors, protease inhibitors, cytokines, lipoprotein lipase and viral envelope proteins, thus plays roles from cell growth, cell differentiation, cell motility, blood coagulation, lipid metabolism to viral infection (1, 2). Heparan Sulfate consists of repeating residues of uronic acid and N‑acetylglucosamine. The uronic acid residues can be sulfated at 2-O position by Heparan Sulfate 2-O Sulfotransferase (HS2ST). The N‑acetylglucosamine residues can be sulfated at N-, 3-O, and 6-O positions by N‑deacetylase/N‑sulfotransferases (NDSTs), Heparan Sulfate 3-O and 6-O sulfotransferases (HS3STs and HS6STs) respectively. However, the reactions catalyzed by these sulfotransferases are normally incomplete on the whole chain of Heparan Sulfate. As a result, Heparan Sulfate displays enormous sequence diversity that allows it to interact with a wide spectrum of proteins differently. Among three HS6STs, HS6ST1 is the first to be cloned (3). Mice deficient of the HS6ST1 homologue gene showed embryonic lethality (4).

  • References:
    1. Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
    2. Esko, J.D. and S.B. Selleck (2002) Annu. Rev. Biochem. 71:435.
    3. Habuchi, H et al. (1998) J. Biol. Chem. 273:9208.
    4. Habuchi, H et al. (2007) J. Biol. Chem. 282:15578.
    5. Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964.
    6. MacRae, I.J. et al. (2000) Biochemistry 39:1613.
    7. Wu, Z.L. et al. (2002) Faseb J. 16:539.
  • Entrez Gene IDs:
    9394 (Human); 50785 (Mouse); 316325 (Rat)
  • Alternate Names:
    DKFZp547H098; EC 2.8.2; EC 2.8.2.-; FLJ25392; heparan sulfate 6-O-sulfotransferase 1; heparan-sulfate 6-sulfotransferase; HS6ST1; HS6ST-1; HS6STheparan-sulfate 6-O-sulfotransferase 1; MGC116899; MGC116901
Related Research Areas

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