Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1052939
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Hepassocin/FGL1
Met1-Ile312
Accession # BAB70690
Met1-Ile312
Accession # BAB70690
Specificity
Detects human Hepassocin/FGL1 in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Hepassocin/FGL1 Antibody
Detection of Hepassocin/FGL1 in HEK293 cells transfected with Human Hepassocin and eGFP cells by Flow Cytometry.
HEK293 cells transfected with Human Hepassocin and eGFP were stained with eGFP and either (A) Mouse Anti-Human Hepassocin/FGL1 Monoclonal Antibody (Catalog # MAB16143) or (B) Mouse IgG2B Isotype Control (Catalog # MAB004) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Intracellular Molecules.
Detection of Human Hepassocin/FGL1 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line, Human Liver. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Hepassocin/FGL1 Monoclonal Antibody (Catalog # MAB16143) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Hepassocin/FGL1 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Human Hepassocin/FGL1 Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 cells transfected with Human Hepassocin and eGFP.
Sample: HEK293 cells transfected with Human Hepassocin and eGFP.
Western Blot
2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line and Human Liver.
Sample: HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line and Human Liver.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Hepassocin/FGL1
Long Name
Fibrinogen Like 1
Alternate Names
FGL1, Fibrinogen-like-protein 1, FREP1
Gene Symbol
FGL1
UniProt
Additional Hepassocin/FGL1 Products
Product Documents for Human Hepassocin/FGL1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Hepassocin/FGL1 Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Flow Cytometry Troubleshooting Guide
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
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- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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