Human Hepassocin/FGL1 Antibody

Catalog #: MAB16143 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB16143-100
MAB16143-SP

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Detection of Hepassocin/FGL1 in HEK293 cells transfected with Human Hepassocin and eGFP cells by Flow Cytometry.

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Human Hepassocin/FGL1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Hepassocin/FGL1 in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1052939

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Hepassocin/FGL1
Met1-Ile312
Accession # BAB70690

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line and Human Liver.

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 cells transfected with Human Hepassocin and eGFP.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Hepassocin/FGL1 in HEK293 cells transfected with Human Hepassocin and eGFP cells by Flow Cytometry. HEK293 cells transfected with Human Hepassocin and eGFP were stained with eGFP and either (A) Mouse Anti-Human Hepassocin/FGL1 Monoclonal Antibody (Catalog # MAB16143) or (B) Mouse IgG_2B Isotype Control (Catalog # MAB004) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Intracellular Molecules.

Western Blot

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Detection of Human Hepassocin/FGL1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line, Human Liver. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Hepassocin/FGL1 Monoclonal Antibody (Catalog # MAB16143) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Hepassocin/FGL1 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hepassocin/FGL1

Hepassocin, also known as hepatocyte-derived fibrinogen-related protein 1 (HFREP-1) and FGL1, is a liver-specific secreted protein belonging to the fibronogen superfamily, whose members share a fibrinogen domain at their C-termini. Hepassocin may play a role in the development of hepatocellular carcinomas.

Long Name

Fibrinogen Like 1

Entrez Gene IDs

2267 (Human); 246186 (Rat); 102118020 (Cynomolgus Monkey)

Alternate Names

FGL1; fibrinogen-like 1; fibrinogen-like protein 1; Fibrinogen-like-protein 1; FREP1; Hepassocin; hepatocellular carcinoma-related sequence; Hepatocyte-derived fibrinogen-related protein 1; HFREP-1; HFREP1MGC12455; HP-041; LFIRE1; LFIRE-1; Liver fibrinogen-related protein 1