Human Hepassocin/FGL1 Antibody

Catalog # Availability Size / Price Qty
MAB16143-100
MAB16143-SP

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Detection of Hepassocin/FGL1 in HEK293 cells transfected with Human Hepassocin and eGFP cells by Flow Cytometry.
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Human Hepassocin/FGL1 Antibody Summary

Specificity
Detects human Hepassocin/FGL1 in direct ELISA.
Source
Monoclonal Mouse IgG2B Clone # 1052939
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Hepassocin/FGL1
Met1-Ile312
Accession # BAB70690
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line and Human Liver.
Flow Cytometry
0.25 µg/106 cells
HEK293 cells transfected with Human Hepassocin and eGFP.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of Hepassocin/FGL1 in HEK293 cells transfected with Human Hepassocin and eGFP cells by Flow Cytometry. HEK293 cells transfected with Human Hepassocin and eGFP were stained with eGFP and either (A) Mouse Anti-Human Hepassocin/FGL1 Monoclonal Antibody (Catalog # MAB16143) or (B) Mouse IgG2B Isotype Control (Catalog # MAB004) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Intracellular Molecules.

Western Blot View Larger

Detection of Human Hepassocin/FGL1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line, Human Liver. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Hepassocin/FGL1 Monoclonal Antibody (Catalog # MAB16143) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Hepassocin/FGL1 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background:

Hepassocin, also known as hepatocyte-derived fibrinogen-related protein 1 (HFREP-1) and FGL1, is a liver-specific secreted protein belonging to the fibronogen superfamily, whose members share a fibrinogen domain at their C-termini. Hepassocin may play a role in the development of hepatocellular carcinomas.

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