Human HIF-2 alpha /EPAS1 Antibody Summary
Accession # Q99814
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human HIF‑2 alpha /EPAS1 by Western Blot. Western blot shows lysates of 786‑O human renal cell adenocarcinoma cell line (HIF‑2 alpha positive), Caki‑2 human clear cell carcinoma epithelial cell line (HIF‑2 alpha negative), and HeLa human cervical epithelial carcinoma cell line (HIF‑2 alpha negative). PVDF Membrane was probed with 1 µg/mL of Human HIF‑2 alpha /EPAS1 Monoclonal Antibody (Catalog # MAB2886) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HIF‑2 alpha /EPAS1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HIF-2 alpha/EPAS1
The hypoxia-inducible transcription factor 2 alpha (HIF-2 alpha ) is stabilized in hypoxic tissue and, similarly to HIF-1 alpha, complexes with Aryl hydrocarbon receptor nuclear translocator (ARNT). Both the HIF-1 and HIF-2 complexes bind hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. HIF-1 and HIF-2 do not appear completely redundant. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the potentially broad impact of gene regulation by both HIF-1 alpha and HIF-2 alpha
Citation for Human HIF-2 alpha /EPAS1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
The von Hippel-Lindau Chuvash mutation promotes pulmonary hypertension and fibrosis in mice.
Authors: Hickey MM, Richardson T, Wang T, Mosqueira M, Arguiri E, Yu H, Yu QC, Solomides CC, Morrisey EE, Khurana TS, Christofidou-Solomidou M, Simon MC
J. Clin. Invest., 2010;120(3):827-39.
Sample Types: Tissue Homogenates
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