Human HIST3H3 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB9448-100
MAB9448-SP

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Detection of Human HIST3H3 by Western Blot.
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Human HIST3H3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HIST3H3 in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2128A
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human HIST3H3 synthetic peptide
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunohistochemistry
3-25 µg/mL
See below
Immunocytochemistry
0.3-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human HIST3H3 antibody by Western Blot. View Larger

Detection of Human HIST3H3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with Sodium (Na) Butyrate. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human HIST3H3 Monoclonal Antibody (Catalog # MAB9448) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HIST3H3 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry HIST3H3 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

HIST3H3 in HeLa Human Cell Line. HIST3H3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human HIST3H3 Monoclonal Antibody (Catalog # MAB9448) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (yellow; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry HIST3H3 antibody in Human Testis by Immunohistochemistry (IHC-P). View Larger

HIST3H3 in Human Testis. HIST3H3 was detected in immersion fixed paraffin-embedded sections of human testis using Rabbit Anti-Human HIST3H3 Monoclonal Antibody (Catalog # MAB9448) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Intracellular Staining by Flow Cytometry Detection of HIST3H3 antibody in HeLa cells antibody by Flow Cytometry. View Larger

Detection of HIST3H3 in HeLa cells by Flow Cytometry. HeLa human cell line was stained with Rabbit anti-Human HIST3H3 Monoclonal Antibody (Catalog # MAB9448, filled histogram) or Normal Rabbit IgG control (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIST3H3

Histones are alkaline proteins which organize nuclear DNA into nucleosomes. Histone H3 is one of four core histones, which exist as dimers to form an octameric histone core. Epigenetic modification of the exposed histone tails by methylation or acetylation is known to regulate transcription in various ways and is known to affect the etiology of a variety of cancers and developmental defects.  Methylation by methyltranserases mediates transcriptional activation (SET1, Chd1), elongation (MLL, WDR5) or silencing (SET7/9, JMJD2A). Expression of H3K4 methyltransferases have been associated with solid tumors such as prostate, ovary, squamous cell carcinoma, hepatocellular carcinoma and cancers of the blood, including T-cell acute lymphoblastic leukemia, acute myeloid leukemia, acute lymphoblastic leukemia, and others.

Long Name
Histone Cluster 3 H3
Entrez Gene IDs
8290 (Human)
Alternate Names
H3 histone family, member T; H3.4; H3/G; H3/gH3.4; H3/T; H3FT; H3FTMGC126888; H3t; H3tMGC126886; HIST3H3; Histone 3, H3; histone cluster 3, H3; histone H3.1t

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Human HIST3H3 Antibody
By Anonymous on 01/28/2022
Application: IHC Sample Tested: Brain tissue Species: Human

Immersion fixed paraffin-embedded sections of human brain. Concentration: 3 ug/mL, 1 hour at room temperature.