Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1079755
View all HLA-E Primary Antibodies »

Product Specifications

Immunogen

HLA-E containing synthetic peptide
Accession # P13747

Specificity

Detects a synthetic peptide around aa 80 in Direct ELISA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human HLA-E Antibody

Detection of HLA-E in Human Tonsil.

HLA-E was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human HLA-E Monoclonal Antibody (Catalog # MAB11547) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human HLA-E Antibody

Application
Recommended Usage

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HLA-E

HLA-E is a class I histocompatibility antigen involved in immune self-nonself discrimination. HLA-E classically binds epitopes from HLA-A, HLA-B, HLA-C and HLA-G signal peptides and serves as a ligand for CD94/NKG2A and CD94/NKG2C receptors on NK and t-cell subsets.  HLA-E down-regulates NK cell cytolytic activity when presented on the cell surface.  It can also restrict non-canonical CD8+ T cells during natural infection with pathogens. HLA-E protein expression in normal human nonlymphoid organs is restricted to endothelial cells.  It is also basally expressed by B and T-lymphocytes, NK cells and macrophages.  TNF-alpha, IL-1beta and IFN-gamma up-regulated cell-surface expression of HLA-EE.

References

  1. Sharpe HR, Bowyer G, Brackenridge S, Lambe T. HLA-E: exploiting pathogen-host interactions for vaccine development. Clin Exp Immunol. 2019 May;196(2):167-177. doi: 10.1111/cei.13292. Epub 2019 Apr 9. PMID: 30968409; PMCID: PMC6468186. 
  2. Lin Z, Bashirova AA, Viard M, Garner L, Quastel M, Beiersdorfer M, Kasprzak WK, Akdag M, Yuki Y, Ojeda P, Das S, Andresson T, Naranbhai V, Horowitz A, McMichael AJ, Hoelzemer A, Gillespie GM, Garcia-Beltran WF, Carrington M. HLA class I signal peptide polymorphism determines the level of CD94/NKG2-HLA-E-mediated regulation of effector cell responses. Nat Immunol. 2023 Jul;24(7):1087-1097. doi: 10.1038/s41590-023-01523-z. Epub 2023 Jun 1. PMID: 37264229; PMCID: PMC10690437. 
  3. Coupel S, Moreau A, Hamidou M, Horejsi V, Soulillou JP, Charreau B. Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation. Blood. 2007 Apr 1;109(7):2806-14. doi: 10.1182/blood-2006-06-030213. PMID: 17179229.

Long Name

Major Histocompatibility Complex, Class I, E

Alternate Names

HLA-6.2, HLAE, QA1

Entrez Gene IDs

3133 (Human)

Gene Symbol

HLA-E

UniProt

P13747 (Human)

Additional HLA-E Products

Product Documents for Human HLA-E Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human HLA-E Antibody

For research use only

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Protocols

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