Human HNF-4 gamma/NR2A2 MAb (Clone N3224)

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HNF‑4 gamma /NR2A2 in Rat Small Intestine.
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Human HNF-4 gamma/NR2A2 MAb (Clone N3224) Summary

Species Reactivity
Monoclonal Mouse IgG2a Clone # N3224
A liquid formulation in physiologic saline with 0.1% NaN3.


Recommended Concentration
Western Blot
Please refer to the PDF datasheet for specifications.
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry HNF‑4?/NR2A2 antibody in Rat Small Intestine by Immunohistochemistry (IHC-P). View Larger

HNF‑4 gamma /NR2A2 in Rat Small Intestine. HNF‑4 gamma /NR2A2 was detected in perfusion fixed paraffin-embedded sections of rat small intestine using HNF‑4 gamma /NR2A2 Monoclonal Antibody (Catalog # PP‑N3224‑00) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Preparation and Storage

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: HNF-4 gamma/NR2A2

Hepatocyte Nuclear Factor 4 alpha (HNF4 alpha; NR2A1) is a member of the orphan nuclear receptor family. HNF4 alpha is expressed in the liver, kidney, intestine and pancreas. Mutation of HNF4 alpha in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4 alpha gene is alternatively spliced and may generate up to nine different isoforms, HNF4 alpha 1 through HNF4 alpha 9. The expression of HNF-4 gamma (NR2A2) is observed in the pancreas, kidney, small intestine, and testes with no expression detected in the liver. The level of HNF-4 gamma is less 10% than that of HNF-4 alpha.

Long Name
Hepatocyte Nuclear Factor 4 gamma
Entrez Gene IDs
3174 (Human)
Alternate Names
hepatocyte nuclear factor 4, gamma; hepatocyte nuclear factor 4-gamma; HNF4 gamma; HNF-4 gamma; HNF4G; HNF-4-gamma; NR2A2; NR2A2Nuclear receptor subfamily 2 group A member 2; NR2A3

Product Datasheets

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Isotype Controls

Mouse IgG2A Isotype Control

Secondary Antibodies

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Human HNF-4 gamma/NR2A2 MAb (Clone N3224)
By Anonymous on 10/23/2022
Application: IHC Sample Tested: Small intestine tissue Species: Human