Human HNF-4 gamma/NR2A2 MAb (Clone N3224)

R&D Systems | Catalog # PP-N3224-00

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # N3224
View all HNF-4 gamma/NR2A2 Primary Antibodies »

Product Specifications

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human HNF-4 gamma/NR2A2 MAb (Clone N3224)

HNF‑4?/NR2A2 antibody in Rat Small Intestine by Immunohistochemistry (IHC-P).

HNF‑4 gamma /NR2A2 in Rat Small Intestine.

HNF‑4 gamma /NR2A2 was detected in perfusion fixed paraffin-embedded sections of rat small intestine using HNF‑4 gamma /NR2A2 Monoclonal Antibody (Catalog # PP‑N3224‑00) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of HNF-4 gamma/NR2A2 by Western Blot

Detection of HNF-4 gamma/NR2A2 by Western Blot

HNF4G promotes resistance to cisplatin in lung adenocarcinoma cells.(A) qRT-PCR and (B) western blot results of HNF4G expression after cisplatin treatment in A549 and HCC827 cells. (C) qRT-PCR results of HNF4G expression in HNF4G overexpression and knockdown cells. (D) Cisplatin sensitivity curves of HNF4G overexpression and knockdown cells. * p < 0.05, ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36923501), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of HNF-4 gamma/NR2A2 by Western Blot

Detection of HNF-4 gamma/NR2A2 by Western Blot

HNF4G promotes resistance to cisplatin in lung adenocarcinoma cells.(A) qRT-PCR and (B) western blot results of HNF4G expression after cisplatin treatment in A549 and HCC827 cells. (C) qRT-PCR results of HNF4G expression in HNF4G overexpression and knockdown cells. (D) Cisplatin sensitivity curves of HNF4G overexpression and knockdown cells. * p < 0.05, ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36923501), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human HNF-4 gamma/NR2A2 MAb (Clone N3224)

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed paraffin-embedded sections of rat small intestine

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using PP-N3224-00 in the following applications:

Formulation, Preparation, and Storage

Formulation

A liquid formulation in physiologic saline with 0.1% NaN3.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: HNF-4 gamma/NR2A2

Hepatocyte Nuclear Factor 4 alpha (HNF4 alpha; NR2A1) is a member of the orphan nuclear receptor family. HNF4 alpha is expressed in the liver, kidney, intestine and pancreas. Mutation of HNF4 alpha in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4 alpha gene is alternatively spliced and may generate up to nine different isoforms, HNF4 alpha 1 through HNF4 alpha 9. The expression of HNF-4 gamma (NR2A2) is observed in the pancreas, kidney, small intestine, and testes with no expression detected in the liver. The level of HNF-4 gamma is less 10% than that of HNF-4 alpha.

Long Name

Hepatocyte Nuclear Factor 4 gamma

Alternate Names

HNF4 gamma, HNF4G, NR2A2

Entrez Gene IDs

3174 (Human)

Gene Symbol

HNF4G

Additional HNF-4 gamma/NR2A2 Products

Product Documents for Human HNF-4 gamma/NR2A2 MAb (Clone N3224)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human HNF-4 gamma/NR2A2 MAb (Clone N3224)

For research use only

Citations for Human HNF-4 gamma/NR2A2 MAb (Clone N3224)

Customer Reviews for Human HNF-4 gamma/NR2A2 MAb (Clone N3224) (1)

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  • Human HNF-4 gamma/NR2A2 MAb (Clone N3224)
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Small intestine tissue
    Species: Human
    Verified Customer | Posted 10/23/2022
    Human HNF-4 gamma/NR2A2 MAb (Clone N3224) PP-N3224-00

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Protocols

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FAQs

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail