Human HOXA1 Antibody

HOXA1 in NTera-2 Human Cell Line. HOXA1 was detected in immersion fixed retinoic acid-treated NTera-2 human testicular embryonic carcinoma cell line using 10 µg/mL Goat Anti-Human HOXA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5014) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human HOXA1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HOXA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5014) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HOXA1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Human HOXA1 by Western Blot Overexpression of RUNX2 induced expression of miR-10a and miR-10b and promoted cell migration and invasion. (A, B) MDA-MB-231 or MCF7 cells were transfected with 4 μg control pcDNA3.1 vector or pcDNA-RUNX2 plasmid for 72 hours. RUNX2, miR-10a or miR-10b expression levels were analyzed by qPCR. (C) RUNX2, HOXA1 and HOXD10 protein levels were assessed by Western blot analysis and normalized to actin. (D, E) Cell migration and invasion ability were assessed by Transwell assay or Matrigel assay, respectively. Black line: 20 μl, Red line: 100 μl (F, G). The quantification result was based on three independent experiments. *: p < 0.05, t-test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25266482), licensed under a CC-BY license. Not internally tested by R&D Systems.