Human HOXA1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human HOXA1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) HOXB1, rhHOXB4, and rhHOXA9 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human HOXA1
    Met1-Asp118
    Accession # P49639
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
HOXA1 in NTera-2 Human Cell Line. HOXA1 was detected in immersion fixed retinoic acid-treated NTera-2 human testicular embryonic carcinoma cell line using 10 µg/mL Goat Anti-Human HOXA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5014) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human HOXA1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HOXA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5014) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HOXA1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HOXA1

HOXA1 (Homeobox A1; also Hox-1F) is a 35 kDa member of the Antp homeobox family of transcription factors. It is expressed in epithelium and is upregulated by E‑cadherin signaling, whereupon it suppresses apoptosis and promotes anchorage dependent growth. Human HOXA1 is 335 amino acids (aa) in length. It contains two poly-His sequences (aa 65-74 and 142-146), a PBX binding segment (aa 204-209) and a DNA binding homeobox domain (aa 229-288). There are multiple splice variants. There is a potential alternate start site at Met6 that may be paired with a nine aa substitution for the C‑terminal 117 aa, or a 19 aa substitution for the C‑terminal 217 amino acids. Over aa 1-118, human HOXA1 shares 92% and 90% aa identity with mouse and canine HOXA1, respectively.

  • Long Name:
    Homeobox A1
  • Entrez Gene IDs:
    3198 (Human); 15394 (Mouse); 25607 (Rat)
  • Alternate Names:
    BSAS; homeo box A1; homeobox A1; Homeobox protein Hox-1F; homeobox protein Hox-A1; Hox 1.6-like protein; HOX A1 homeodomain protein; HOX1; HOX1F; HOX1Fhomeobox 1F; HOXA1; lab-like protein; MGC45232
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