Human HOXA1 Antibody

Catalog # Availability Size / Price Qty
AF5014
AF5014-SP
HOXA1 in NTera-2 Human Cell Line.
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Human HOXA1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HOXA1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) HOXB1, rhHOXB4, and rhHOXA9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human HOXA1
Met1-Asp118
Accession # P49639
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry HOXA1 antibody in NTera-2 Human Cell Line by Immunocytochemistry (ICC). View Larger

HOXA1 in NTera-2 Human Cell Line. HOXA1 was detected in immersion fixed retinoic acid-treated NTera-2 human testicular embryonic carcinoma cell line using 10 µg/mL Goat Anti-Human HOXA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5014) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot Detection of Human HOXA1 antibody by Western Blot. View Larger

Detection of Human HOXA1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HOXA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5014) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HOXA1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HOXA1

HOXA1 (Homeobox A1; also Hox-1F) is a 35 kDa member of the Antp homeobox family of transcription factors. It is expressed in epithelium and is upregulated by E‑cadherin signaling, whereupon it suppresses apoptosis and promotes anchorage dependent growth. Human HOXA1 is 335 amino acids (aa) in length. It contains two poly-His sequences (aa 65-74 and 142-146), a PBX binding segment (aa 204-209) and a DNA binding homeobox domain (aa 229-288). There are multiple splice variants. There is a potential alternate start site at Met6 that may be paired with a nine aa substitution for the C‑terminal 117 aa, or a 19 aa substitution for the C‑terminal 217 amino acids. Over aa 1-118, human HOXA1 shares 92% and 90% aa identity with mouse and canine HOXA1, respectively.

Long Name
Homeobox A1
Entrez Gene IDs
3198 (Human); 15394 (Mouse); 25607 (Rat)
Alternate Names
BSAS; homeo box A1; homeobox A1; Homeobox protein Hox-1F; homeobox protein Hox-A1; Hox 1.6-like protein; HOX A1 homeodomain protein; HOX1; HOX1F; HOX1Fhomeobox 1F; HOXA1; lab-like protein; MGC45232

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody

WB,ELISA

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