Human ICOS Antibody

Catalog # Availability Size / Price Qty
MAB69753-100
MAB69753-SP
Detection of ICOS in HEK293 Human Cell Line Transfected with Human ICOS by Flow Cytometry.
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Human ICOS Antibody Summary

Species Reactivity
Human
Specificity
Detects human ICOS in direct ELISAs.
Source
Monoclonal Mouse IgG3 Clone # 669214
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human ICOS and mouse myeloma cell line NS0-derived recombinant human ICOS cocktail
Glu21-Phe141
Accession # Q9Y6W8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Blockade of Receptor-ligand Interaction
In a functional Flow Cytometry test, 2.5 μg/mL of Mouse Anti-Human ICOS Monoclonal Antibody completely blocks binding of biotinylated recombinant Human B7-H2 (Catalog # 8206-B7) to Human ICOS-transfected HEK293 cells
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of ICOS antibody in HEK293 Human Cell Line Transfected with Human ICOS antibody by Flow Cytometry. View Larger

Detection of ICOS in HEK293 Human Cell Line Transfected with Human ICOS by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human ICOS was stained with Mouse Anti-Human ICOS Monoclonal Antibody (Catalog # MAB69753, filled histogram) or isotype control antibody (Catalog # MAB007, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Blockade of Receptor-ligand Interaction Human B7-H2 Binding to ICOS-transfected HEK293 Human Cell Line is Blocked by Human ICOS Antibody. View Larger

Human B7-H2 Binding to ICOS-transfected HEK293 Human Cell Line is Blocked by Human ICOS Antibody. In a functional Flow Cytometry test, biotinylated recombinant Human B7-H2 (Catalog # 8206-B7, 5 ng/mL) binds to Human ICOS-transfected HEK293 cells (black dotted line). Binding is completely blocked (filled histogram) by 2.5 µg/mL of Mouse Anti-Human ICOS Monoclonal Antibody (Catalog # MAB69753). Mouse IgG3 isotype (Catalog # MAB007) at 2.5 µg/mL was used as a control (blue line). Cells were stained with Sterptavidin-APC (Catalog # F0050).

Blockade of Receptor-ligand Interaction ICOS binding to B7-H2 blocked by Human ICOS Antibody. View Larger

ICOS binding to B7-H2 blocked by Human ICOS Antibody. In a Surface Plasmon Resonance test, Recombinant Human B7-H2 (Catalog # 8206-B7) coated at 30 ug/mL binds to Recombinant Human B7-H7 (Catalog # 8084-B7, 25 µg/mL (green line). Binding is completely blocked (orange line) by 50 µg/mL of Mouse Anti-Human ICOS Monoclonal Antibody (Catalog # MAB69753). Non-blocking anti-ICOS antibody (Clone 669244) was used as control and did not affect ICOS binding to B7-H2 (blue line).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICOS

Inducible costimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4, and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is up-regulated within approximately 24-48 hours of activation on Th primed cells. B7-H2, a member of the B7 family of costimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

References
  1. Aicher, A. et al. (2000) J. Immunol. 164:4689.
  2. Coyle, A.J. et al. (2000) Immunity 13:95.
  3. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  4. Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
  5. Hutloff, A. et al. (1999) Nature 397:263.
  6. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
  7. Yoshinaga, S.K. et al. (1999) Nature 402:827.
Long Name
Inducible Co-stimulator
Entrez Gene IDs
29851 (Human); 54167 (Mouse); 102116512 (Cynomolgus Monkey)
Alternate Names
Activation-inducible lymphocyte immunomediatory molecule; AILIM; AILIMCD278; CD278 antigen; CD278; CRP-1; CVID1; ICOS; inducible costimulator; inducible T-cell costimulator; inducible T-cell co-stimulator; MGC39850

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