Human IFN-alpha Antibody

Catalog # Availability Size / Price Qty
IFN‑ alpha  in Human PBMCs and MCF-7 Human Cell Line.
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Product Details
Supplemental Products

Human IFN-alpha Antibody Summary

Species Reactivity
Detects human IFN‑ alpha 2a,4a,6,7,8,10,16, and 17 in direct ELISAs.
Monoclonal Mouse IgG1 Clone # 995514
Protein A or G purified from hybridoma culture supernatant
HEK293 human embryonic kidney cell line transfected with human IFN‑ alpha 2
Accession # P01563
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry IFN-a antibody in Human PBMCs and MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

IFN‑ alpha in Human PBMCs and MCF-7 Human Cell Line. IFN-a was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs; left panel, positive stain) and MCF-7 human breast cancer cell line (right panel, negative stain) using Mouse Anti-Human IFN-a Monoclonal Antibody (Catalog # MAB93454) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha

Interferon-alpha (IFN-alpha or IFN-alpha ), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. Many IFN-alpha subtypes exist, IFN-alpha 1 to IFN alpha -21. The major subtypes identified are IFN-alpha 1, IFN-alpha 2, IFN-alpha 8, IFN-alpha 10, IFN-alpha 14 and IFN-alpha 21. MAB93454 detects human IFN-alpha 2a,4a,6,7,8,10,16, and 17 in direct ELISA.

Long Name
Interferon alpha
Entrez Gene IDs
3440 (Human)
Alternate Names
IFNA; IFNA2; IFNA2b; IFNalpha; IFN-alpha; IFN-alphaA; INFA2; interferon alpha 2; LeIF D

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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