Human IFN-alpha Antibody (995514)

Name: Human IFN-alpha Antibody (995514)
Brand: R&D Systems
SKU: MAB93454
Price: 159 USD
Availability: InStock

R&D Systems | Catalog # MAB93454

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Product Specifications
Scientific Data
Applications
Preparation & Storage
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Background
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 995514

View all IFN-alpha Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human IFN‑ alpha 2
Met1-Glu188
Accession # P01563

Specificity

Detects human IFN‑ alpha 2a,4a,6,7,8,10,16, and 17 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human IFN-alpha Antibody (995514)

IFN‑ alpha in Human PBMCs and MCF-7 Human Cell Line.

IFN-a was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs; left panel, positive stain) and MCF-7 human breast cancer cell line (right panel, negative stain) using Mouse Anti-Human IFN-a Monoclonal Antibody (Catalog # MAB93454) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human IFN-alpha Antibody (995514)

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IFN-alpha

Interferon-alpha (IFN-alpha or IFN-alpha ), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. Many IFN-alpha subtypes exist, IFN-alpha 1 to IFN alpha -21. The major subtypes identified are IFN-alpha 1, IFN-alpha 2, IFN-alpha 8, IFN-alpha 10, IFN-alpha 14 and IFN-alpha 21. MAB93454 detects human IFN-alpha 2a,4a,6,7,8,10,16, and 17 in direct ELISA.