Human IFN-beta Antibody

Catalog # Availability Size / Price Qty
AF814
AF814-SP
IFN‑ beta  Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ beta  Antibody.
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Product Details
Citations (5)
FAQs
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Human IFN-beta Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFN-beta in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse IFN‑ beta 1, recombinant human (rh) IFN-alpha 2, rhIFN‑ gamma, recombinant cotton rat (rcr) IFN-alpha  and rcrIFN‑ gamma is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IFN-beta
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IFN-beta
Neutralization
Measured by its ability to neutralize IFN‑ beta inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND50) is typically 0.05-0.2 µg/mL in the presence of 50 pg/mL Recombinant Human IFN‑ beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization IFN‑ beta  Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ beta  Antibody. View Larger

IFN‑ beta Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ beta  Antibody. Recombinant Human IFN‑ beta (Catalog # 8499-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN‑ beta (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IFN‑ beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF814). The ND50 is typically 0.05-0.2 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-beta

IFN-beta (interferon beta; also fibroblast IFN) is a secreted, monomeric 23-24 kDa member of the alpha/beta interferon family of molecules. It can be produced by almost all cell types in response to bacterial DNA or viral double-stranded RNA and is abundantly secreted by circulating plasmacytoid DCs. IFN-beta drives monocytic transformation into DCs, and it appears to stimulate a B cell switch from from IgM to IgG secretion. Mature human IFN-beta is 166 amino acids (aa) in length (aa 22-187) and contains one phosphorylation site at Ser140. There is one potential alternative start site at Met22. Full-length human IFN-beta (aa 22-187) shares 47% aa identity with mouse IFN-beta.

Long Name
Interferon beta
Entrez Gene IDs
3456 (Human); 15977 (Mouse); 24481 (Rat)
Alternate Names
Fibroblast interferon; IFBIFNB; IFF; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956

Product Datasheets

Citations for Human IFN-beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Dobrava-Belgrade hantavirus from Germany shows receptor usage and innate immunity induction consistent with the pathogenicity of the virus in humans.
    Authors: Popugaeva E, Witkowski PT, Schlegel M, Ulrich RG, Auste B, Rang A, Kruger DH, Klempa B
    PLoS ONE, 2012;7(4):e35587.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  2. Human cytomegalovirus IE1 protein elicits a type II interferon-like host cell response that depends on activated STAT1 but not interferon-gamma.
    Authors: Knoblach T, Grandel B, Seiler J, Nevels M, Paulus C
    PLoS Pathog., 2011;7(4):e1002016.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  3. The LIM-homeodomain transcription factor LMX1B regulates expression of NF-kappa B target genes.
    Authors: Rascle A, Neumann T, Raschta AS, Neumann A, Heining E, Kastner J, Witzgall R
    Exp. Cell Res., 2009;315(1):76-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  4. TLR3 can directly trigger apoptosis in human cancer cells.
    Authors: Salaun B, Coste I, Rissoan MC, Lebecque SJ, Renno T
    J. Immunol., 2006;176(8):4894-901.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  5. Inhibition of human immunodeficiency virus type 1 replication by Z-100, an immunomodulator extracted from human-type tubercle bacilli, in macrophages.
    Authors: Emori Y, Ikeda T, Ohashi T, Masuda T, Kurimoto T, Takei M, Kannagi M
    J. Gen. Virol., 2004;85(0):2603-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut

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