Human IFN-gamma Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF285
IFN‑ gamma  in Human PBMCs.
2 Images
Product Details
Citations (15)
FAQs
Supplemental Products
Reviews

Human IFN-gamma Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFN-gamma in ELISAs and Western blots. In sandwich immunoassays, less than 3% cross-reactivity with recombinant rhesus macaque IFN‑ gamma is observed and less than 0.05% cross-reactivity with recombinat rat IFN-gamma, recombinant feline IFN-gamma, recombinant equine IFN-gamma, recombinant cotton rat IFN-gamma, recombinant canine IFN-gamma, recombinant porcine IFN-gamma, and recombinant mouse IFN-gamma is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IFN-gamma (R&D Systems, Catalog # 285-IF)
Met1-Gln144
Accession # Q14609
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IFN-gamma (Catalog # 285-IF)
Immunocytochemistry
5-15 µg/mL
See below

Human IFN-gamma Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human IFN‑ gamma Antibody (Catalog # MAB2852)

Standard: Recombinant Human IFN-gamma Protein (Catalog # 285-IF)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry IFN‑? antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

IFN‑ gamma in Human PBMCs. IFN‑ gamma was detected in immersion fixed PMA- and ionomycin-stimulated human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human IFN‑ gamma Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF285) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry IFN-? antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

IFN‑ gamma in Human PBMCs. IFN-gamma was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA, ionomyocin, and monensin using Human IFN-gamma Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF285) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma

Interferon-gamma (IFN-gamma, IFNG), also known as type II or Immune Interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa molecular weight variably glycosylated subunits. Glycosylation of IFN-gamma at sites Asn25 and Asn97 is critical for protease resistance. It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59-64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key function in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.

Long Name
Interferon gamma
Entrez Gene IDs
3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

Product Datasheets

You must select a language.

x

Citations for Human IFN-gamma Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Defining T cell receptor repertoires using nanovial-based affinity and functional screening
    Authors: Doyeon Koo, Zhiyuan Mao, Robert Dimatteo, Natalie Tsubamoto, Miyako Noguchi, Jami McLaughlin et al.
    bioRxiv
  2. Immunogenic epitope panel for accurate detection of non-cross-reactive T cell response to SARS-CoV-2
    Authors: A Titov, R Shaykhutdi, OV Shcherbako, YV Serdyuk, SA Sheetikov, KV Zornikova, AV Maleeva, A Khmelevska, DV Dianov, NT Shakirova, DB Malko, M Shkurnikov, S Nersisyan, A Tonevitsky, E Khamaganov, AV Ershov, EY Osipova, RV Nikolaev, DE Pershin, VA Vedmedskia, M Maschan, VR Ginanova, GA Efimov
    JCI Insight, 2022-05-09;7(9):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Detection
  3. Hydrogel based protein biochip for parallel detection of biomarkers for diagnosis of a Systemic Inflammatory Response Syndrome (SIRS) in human serum
    Authors: A Stumpf, T Brandstett, J Hübner, J Rühe
    PLoS ONE, 2019-12-02;14(12):e0225525.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Detection
  4. Decreased allergy incidence in children supplemented with E. coli O83:K24:H31 and its possible modes of action
    Authors: J Hrdý, K Vlasáková, V ?erný, L Súkeníková, O Novotná, P Petrásková, K Boráková, R Lodinová-Ž, L Kolá?ová, L Prokešová
    Eur. J. Immunol., 2018-10-21;0(0):.
    Species: Human
    Sample Types: Plasma
  5. Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma
    Authors: E Brencicova, AL Jagger, HG Evans, M Georgouli, A Laios, S Attard Mon, G Mehra, J Spencer, AA Ahmed, S Raju-Kanki, LS Taams, SS Diebold
    PLoS ONE, 2017-04-14;12(4):e0175712.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  6. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  7. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
    Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
    Clin. Chem., 2007-11-01;53(11):2010-2.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  8. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007-10-22;329(1):214-8.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development
  9. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007-02-08;109(10):4272-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  10. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
    Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
    AIDS, 2007;21(10):1263-72.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  11. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006-06-16;120(2):220-31.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  12. Fractalkine reduces N-methyl-d-aspartate-induced calcium flux and apoptosis in human neurons through extracellular signal-regulated kinase activation.
    Authors: Deiva K, Geeraerts T, Salim H, Leclerc P, Hery C, Hugel B, Freyssinet JM, Tardieu M
    Eur. J. Neurosci., 2004-12-01;20(12):3222-32.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  13. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
    Authors: Copeland S, Siddiqui J, Remick D
    J. Immunol. Methods, 2004-01-01;284(1):99-106.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Array Development
  14. CD1d-mediated Recognition of an alpha -Galactosylceramide by Natural Killer T Cells Is Highly Conserved through Mammalian Evolution
    Authors: Laurent Brossay, Mariacristina Chioda, Nicolas Burdin, Yasuhiko Koezuka, Giulia Casorati, Paolo Dellabona et al.
    J. Exp. Med
  15. Potential Limitations of the NSG Humanized Mouse as a Model System to Optimize Engineered Human T cell Therapy for Cancer
    Authors: Erik M. Alcantar-Orozco, Hannah Gornall, Vania Baldan, Robert E. Hawkins, David E. Gilham
    Human Gene Therapy Methods

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human IFN-gamma Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Human IFN-gamma Biotinylated Antibody and earn rewards!

Have you used Human IFN-gamma Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review