Human IFN‑ gamma Biotinylated Antibody

Name: Human IFN‑ gamma Biotinylated Antibody
Brand: R&D Systems
SKU: BAF285
Price: 589 USD
Availability: InStock

R&D Systems | Catalog # BAF285

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Citations (43)

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair), Immunocytochemistry

Cited:

Immunohistochemistry-Frozen, Immunocytochemistry, Array Development, Bioassay, Capture Assay, ELISA Capture, ELISA Detection, ELISA Development, ELISA Development (Detection), Luminex Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all IFN-gamma Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IFN-gamma (R&D Systems, Catalog # 285-IF)
Met1-Gln144
Accession # Q14609

Specificity

Detects human IFN-gamma in ELISAs and Western blots. In sandwich immunoassays, less than 3% cross-reactivity with recombinant rhesus macaque IFN‑ gamma is observed and less than 0.05% cross-reactivity with recombinat rat IFN-gamma, recombinant feline IFN-gamma, recombinant equine IFN-gamma, recombinant cotton rat IFN-gamma, recombinant canine IFN-gamma, recombinant porcine IFN-gamma, and recombinant mouse IFN-gamma is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human IFN‑ gamma Biotinylated Antibody

IFN‑ gamma in Human PBMCs.

IFN‑ gamma was detected in immersion fixed PMA- and ionomycin-stimulated human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human IFN‑ gamma Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF285) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

IFN‑ gamma in Human PBMCs.

IFN-gamma was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA, ionomyocin, and monensin using Human IFN-gamma Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF285) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human IFN‑ gamma Biotinylated Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA, ionomycin, and monensin

Western Blot

0.1 µg/mL
Sample: Recombinant Human IFN-gamma (Catalog # 285-IF)

Human IFN-gamma Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:

Capture Reagent: Human IFN‑ gamma Antibody (Catalog # MAB2852)
Standard: Recombinant Human IFN-gamma Protein (Catalog # 285-IF)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IFN-gamma

Interferon-gamma (IFN-gamma, IFNG), also known as type II or Immune Interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa molecular weight variably glycosylated subunits. Glycosylation of IFN-gamma at sites Asn25 and Asn97 is critical for protease resistance. It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59-64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key function in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.