Detects human IFN-gamma in ELISAs and Western blots. In sandwich immunoassays, less than 3% cross-reactivity with recombinant rhesus macaque IFN-gamma is observed and less than 0.05% cross-reactivity with recombinat rat IFN-gamma, recombinant feline IFN-gamma, recombinant equine IFN-gamma, recombinant cotton rat IFN-gamma, recombinant canine IFN-gamma, recombinant porcine IFN-gamma, and recombinant mouse IFN-gamma is observed.
Polyclonal Goat IgG
E. coli-derived recombinant human IFN-gamma (R&D Systems, Catalog # 285-IF) Met1-Gln144 Accession # Q14609
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
IFN‑ gamma in Human PBMCs.
IFN‑ gamma was detected in immersion fixed PMA- and ionomycin-stimulated human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human IFN‑ gamma Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF285) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
IFN‑ gamma in Human PBMCs.
IFN‑ gamma was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA, ionomyocin, and monensin using Human IFN‑ gamma Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF285) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits. It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59-64% with bovine, canine, equine, feline, and porcine IFN-gamma, and 37-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.
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