Human IFN-gamma R1/CD119 Antibody
Human IFN-gamma R1/CD119 Antibody Summary
IFN-gamma R2 is observed.
Accession # P15260
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma R1/CD119 Antibody. Recombinant Human IFN-gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IFN-gamma (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IFN-gamma R1/CD119 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF673). The ND50 is typically 0.5-2 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-gamma R1/CD119
The high-affinity IFN-gamma receptor complex is made up of two type I membrane proteins, IFN-gamma R1 (IFN-gamma R alpha ) and IFN-gamma R2 (IFN-gamma R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% overall sequence identity. IFN-gamma R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma R1 cDNA encodes a 499 amino acid (aa) residue protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma R1 share 52% amino acid sequence similarity and bind IFN-gamma in a species-specific manner. IFN-gamma R1 is constitutively expressed in most cell types. Soluble IFN-gamma R1 that binds IFN-gamma has been detected in biological fluids. The recombinant soluble IFN-gamma R1 produced at R&D Systems has been shown to bind IFN-gamma with high affinity and is a potent IFN-gamma antagonist.
Citations for Human IFN-gamma R1/CD119 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Inhibition of interferon-signalling halts cancer-associated fibroblast-dependent protection of breast cancer cells from chemotherapy
Authors: RV Broad, SJ Jones, MC Teske, LM Wastall, AM Hanby, JL Thorne, TA Hughes
British Journal of Cancer, 2021-01-04;0(0):.
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC
Inborn Errors of RNA Lariat Metabolism in Humans with Brainstem Viral Infection
Authors: SY Zhang, NE Clark, CA Freije, E Pauwels, AJ Taggart, S Okada, H Mandel, P Garcia, MJ Ciancanell, A Biran, FG Lafaille, M Tsumura, A Cobat, J Luo, S Volpi, B Zimmer, S Sakata, A Dinis, O Ohara, EJ Garcia Rei, K Dobbs, M Hasek, SP Holloway, K McCammon, SA Hussong, N DeRosa, CE Van Skike, A Katolik, L Lorenzo, M Hyodo, E Faria, R Halwani, R Fukuhara, GA Smith, V Galvan, MJ Damha, S Al-Muhsen, Y Itan, JD Boeke, LD Notarangel, L Studer, M Kobayashi, L Diogo, WG Fairbrothe, L Abel, BR Rosenberg, PJ Hart, A Etzioni, JL Casanova
Sample Types: Cell Culture Supernates
Applications: ELISA Capture
Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
Authors: Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G
Sample Types: Whole Cells
Expression of interferon receptors in pancreatic cancer: identification of a novel prognostic factor.
Authors: Saidi RF, Williams F, Silberberg B, Mittal VK, ReMine SG, Jacobs MJ
Sample Types: Whole Tissue
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