Human IL-12 R beta 2 Antibody

Catalog # Availability Size / Price Qty
MAB19591
MAB19591-SP

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Detection of Human IL‑12 R beta 2 by Western Blot.
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Human IL-12 R beta 2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-12 R beta 2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human IL‑12 R beta 1 or recombinant mouse IL-12 R beta 1 is observed.
Source
Monoclonal Mouse IgG2A Clone # 622509
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-12 R beta 2
Lys24-Cys622
Accession # Q99665
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human IL-12 R beta 2 antibody by Western Blot. View Larger

Detection of Human IL‑12 R beta 2 by Western Blot. Western blot shows lysates of human CD4+in vitrodifferentiated Th1 cells. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human IL-12 R beta 2 Monoclonal Antibody (Catalog # MAB19591) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-12 R beta 2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12 R beta 2

IL-12 R beta 2 is a type I cytokine receptor family protein that associates with IL 12 R beta 1 to form a functional high affinity receptor complex for IL-12. The extracellular region of IL 12 R beta 2 contains five fibronectin type 3 domains. The cytoplasmic region is alternatively spliced, giving rise to two isoforms. IL-12 R beta 2 is up‑regulated in Th1 cells during inflammatory responses. The extracellular regions of human and mouse IL‑12 R beta 2 share 69% amino acid sequence identity.

Long Name
Interleukin 12 Receptor beta 2
Entrez Gene IDs
3595 (Human); 16162 (Mouse)
Alternate Names
IL-12 R beta 2; IL-12 receptor beta 2; IL-12 receptor subunit beta-2; IL12R beta 2; IL-12R subunit beta-2; IL12RB2; IL-12Rb2; IL-12R-beta-2; interleukin 12 receptor, beta 2; interleukin-12 receptor beta-2 chain; interleukin-12 receptor subunit beta-2

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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