Human IL-13 Antibody Summary
Accession # AAK53823
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑13 and Neutralization by Human IL‑13 Antibody. Recombinant Human IL-13 (213-ILB) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-213-NA). The ND50 is typically 0.5‑3 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature human IL-13 shares 57%, 59%, and 94% amino acid sequence identity with mouse, rat, and rhesus macaque IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of pro-inflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is over-expressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).
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- Joshi, B.H. et al. (2006) Vitam. Horm. 74:479.
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Citations for Human IL-13 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 5
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Dual vaccination against IL-4 and IL-13 protects against chronic allergic asthma in mice
Authors: E Conde, R Bertrand, B Balbino, J Bonnefoy, J Stackowicz, N Caillot, F Colaone, S Hamdi, R Houmadi, A Loste, JBJ Kamphuis, F Huetz, L Guillemina, N Gaudenzio, A Mougel, D Hardy, JN Snouwaert, BH Koller, V Serra, P Bruhns, G Grouard-Vo, LL Reber
Nature Communications, 2021-05-11;12(1):2574.
Sample Types: Recombinant Proteins
Applications: Western Blot
Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
Eur. J. Immunol., 2008-04-01;38(4):1012-23.
Sample Types: Whole Cells
Histological characteristics and altered expression of interleukins (IL) IL-13 and IL-15 in endometria of levonorgestrel users with different uterine bleeding patterns.
Authors: Rhoton-Vlasak A, Hardt N
Fertil. Steril., 2005-03-01;83(3):659-65.
Sample Types: Whole Tissue
Differential expression of interleukins IL-13 and IL-15 in normal ovarian tissue and ovarian carcinomas.
Authors: Ripley D, Shoup B, Majewski A
Gynecol. Oncol., 2004-03-01;92(3):761-8.
Sample Types: Whole Tissue
Human lung myofibroblasts as effectors of the inflammatory process: the common receptor gamma chain is induced by Th2 cytokines, and CD40 ligand is induced by lipopolysaccharide, thrombin and TNF-alpha.
Authors: Doucet C, Giron-Michel J, Canonica GW, Azzarone B
Eur. J. Immunol., 2002-09-01;32(9):2437-49.
Sample Types: Whole Cells
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