Human IL-13 Antibody

(5 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-13 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross‑reactivity with recombinant rhesus monkey IL-13 is observed, approximately 25% cross‑reactivity with recombinant canine IL-13 is observed, and approximately 15% cross‑reactivity with recombinant rat IL-13 is observed and less than 5% cross‑reactivity with recombinant mouse IL-13 and recombinant cotton rat IL-13 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human IL-13
    Gly21-Asn132
    Accession # AAK53823
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human IL‑13 (Catalog # 213-ILB)
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Intracellular Staining by Flow Cytometry
    5 µg/106 cells
    Human IL-13 transfected NS0 cells fixed with paraformaldehyde and permeabilized with saponin
  • Neutralization
    Measured by its ability to neutralize IL‑13-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.5-3 µg/mL in the presence of 10 ng/mL Recombinant Human IL‑13.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑13 and Neutralization by Human IL‑13 Antibody. Recombinant Human IL‑13 (Catalog # 213-ILB) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-213-NA). The ND50 is typically 0.5-3 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-13

IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature human IL-13 shares 57%, 59%, and 94% amino acid sequence identity with mouse, rat, and rhesus macaque IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of pro-inflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is over-expressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).

  • References:
    1. Wills-Karp, M. (2004) Immunol. Rev. 202:175.
    2. Nakajima H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265.
    3. McKenzie, A.N. et al. (1993) Proc. Natl. Acad. Sci. USA 90:3735.
    4. Moy, F.J. et al. (2001) J. Mol. Biol. 310:219.
    5. Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231.
    6. Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
    7. Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529.
    8. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
    9. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
    10. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
    11. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
    12. Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387.
    13. Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun. 360:464.
    14. Tabata, Y. et al. (2007) J. Immunol. 177:7905.
    15. Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
    16. Joshi, B.H. et al. (2006) Vitam. Horm. 74:479.
    17. Andrews, A-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.
  • Long Name:
    Interleukin 13
  • Entrez Gene IDs:
    3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)
  • Alternate Names:
    ALRHMGC116789; BHR1interleukin-13; IL13; IL-13; IL-13MGC116788; interleukin 13; MGC116786; NC30; P600
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
    Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
    Eur. J. Immunol., 2008;38(4):1012-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Histological characteristics and altered expression of interleukins (IL) IL-13 and IL-15 in endometria of levonorgestrel users with different uterine bleeding patterns.
    Authors: Rhoton-Vlasak A, Hardt N
    Fertil. Steril., 2005;83(3):659-65.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  3. Differential expression of interleukins IL-13 and IL-15 in normal ovarian tissue and ovarian carcinomas.
    Authors: Ripley D, Shoup B, Majewski A
    Gynecol. Oncol., 2004;92(3):761-8.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. Differential expression of interleukins IL-13 and IL-15 in normal ovarian tissue and ovarian carcinomas.
    Authors: Ripley D, Shoup B, Majewski A
    Gynecol. Oncol., 2004;92(3):761-8.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  5. Human lung myofibroblasts as effectors of the inflammatory process: the common receptor gamma chain is induced by Th2 cytokines, and CD40 ligand is induced by lipopolysaccharide, thrombin and TNF-alpha.
    Authors: Doucet C, Giron-Michel J, Canonica GW, Azzarone B
    Eur. J. Immunol., 2002;32(9):2437-49.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

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Goat IgG (H+L) APC-conjugated Antibody

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Goat IgG Horseradish Peroxidase-conjugated Antibody

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Donkey Anti-Goat IgG Antibody

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Goat IgG VisUCyte HRP Polymer

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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Average Rating: 3 (Based on 1 review)

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Summary

ApplicationELISA
Sample Tested3T3-L1 mouse embryonic fibroblast adipose-like cell line
SpeciesMouse

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