Human IL-17E/IL-25 Antibody

Catalog # Availability Size / Price Qty
AF1258
AF1258-SP
Human IL-17E/IL-25 Antibody in Data
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Product Details
Citations (1)
FAQs
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Reviews (1)

Human IL-17E/IL-25 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-17E in direct ELISAs and Western blots.  In direct ELISAs, less than 60% cross-reactivity with recombinant mouse IL‑17E is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL-17E (R&D Systems, Catalog # 1258-IL)
Tyr33-Gly177
Accession # Q9H293
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL-17E/IL-25 (Catalog # 1258-IL)
Neutralization
Measured by its ability to neutralize IL‑17E/IL‑25-induced CXCL1/GRO alpha secretion in the HT‑29 human colon adenocarcinoma cell line. The Neutralization Dose (ND50) is typically 0.04-0.24 ug/mL in the presence of 5 ng/ml Recombinant Human IL‑17E/IL‑25.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell CXCL1/GRO Alpha Secretion Induced by IL‑17E/IL‑25 and Neutralization by Human IL‑17E/IL‑25 Antibody. View Larger

Cell CXCL1/GRO Alpha Secretion Induced by IL‑17E/IL‑25 and Neutralization by Human IL‑17E/IL‑25 Antibody. Recombinant Human IL-17E/ IL-25 induces CXCL1/GRO alpha secretion in HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human CXCL1/GRO alpha Duoset (Catalog # DY275). Under these conditions, CXCL1/GRO alpha secretion elicited by IL-17E/IL-25 is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-17E/IL-25 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1258). The ND50is typically 0.04-0.24 ug/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17E/IL-25

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2).

Human IL-17E cDNA encodes a 177 amino acid (aa) residues precursor protein with a putative 32 aa signal peptide (3). A second isoform of human IL-17E encoding a 161 aa precursor protein also exists (4). The two isoforms differ in their signal peptide sequences. Mature human IL-17E shares 76% aa sequence identity with mature mouse IL-17E. Human IL-17E also shares from 25% to 36% aa sequence identity with the other human IL-17 family members. IL-17E expression was detected at very low levels by PCR in various peripheral tissues including brain, kidney, lung, prostate, testis, adrenal gland spinal cord and trachea (3). IL-17E binds and activates IL‑17 B Receptor (IL-17B R) (alternatively known as IL-17 Rh1, IL-17E R, and EVI27) (3), which is expressed in kidney and liver, and at lower levels in brain, testis and other endocrine tissues. The expression of IL-17B R is up regulated under inflammatory conditions. Ligation of IL-17E to IL-17 RB induces activation of nuclear factor kappa-B and stimulates the production of the proinflamatory cytokine IL-8 (3). IL-17 has also been found to promote the expression of the prototypical Th2 genes (4, 5).

References
  1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
  2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
  3. Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
  4. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
  5. Pan, G. et al. (2001) J. Immunol. 167:6569.
Long Name
Interleukin 17E
Entrez Gene IDs
64806 (Human); 140806 (Mouse); 501996 (Rat)
Alternate Names
IL17E; IL-17E; IL25; IL-25; interleukin 25; Interleukin-17E; interleukin-25

Product Datasheets

Citation for Human IL-17E/IL-25 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. IL-25 promotes Th2 bias by upregulating IL-4 and IL-10 expression of decidual ??T cells in early pregnancy.
    Authors: Yuan Zhang, Ying Wang, Ming-Qing Li, Jie Duan, Deng-Xuan Fan, Li-Ping Jin
    Experimental and Therapeutic Medicine, 2018;0(0):1792-0981.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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Isotype Controls

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Secondary Antibodies

Reviews for Human IL-17E/IL-25 Antibody

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Human IL-17E/IL-25 Antibody
By Anonymous on 06/09/2020
Application: ELISA Sample Tested: Serum and Plasma Species: Human

We used this antibody in an in-house ELISA along with mAb (1258) and protein (1258-IL-025) to quantify IL-17E in human serum and plasma. This combination detected IL-17E in our samples efficiently.