Human IL-18 R beta /IL-1 R7 Antibody

Catalog # Availability Size / Price Qty
MAB1181
MAB1181-SP
IFN-gamma  secretion Induced by IL-18/IL1F4 and Neutralization by Human IL-18 R beta /IL-1 R7 Antibody.
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Citations (4)
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Human IL-18 R beta /IL-1 R7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑18 R beta /IL‑1 R7 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse IL-18 R beta, recombinant human (rh) IL-18 R, rhIL-1 RI, rhIL-1 RII, rhIL-1 RAcP, and rhIL-1 Rrp2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 132016
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL‑18 R beta /IL‑1 R7
Met1-Arg356
Accession # O95256
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL‑18 R beta /IL‑1 R7 Fc Chimera (Catalog # 118-AP)
Neutralization
Measured by its ability to neutralize IL-18/IL-1F4-induced IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line. Novick, D. et al. (1999) Immunity 10:127. The Neutralization Dose (ND50) is typically 0.3-1.0 μg/mL in the presence of 40 ng/mL Recombinant Human IL-18/IL-1F4 and 20 ng/mL Recombinant Human TNF-alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IFN-gamma  secretion Induced by IL-18/IL1F4 and Neutralization by Human IL-18 R beta /IL-1 R7 Antibody. View Larger

IFN-gamma secretion Induced by IL-18/IL1F4 and Neutralization by Human IL-18 R beta /IL-1 R7 Antibody. In the presence of Recombinant Human TNF-alpha (20 ng/mL, Catalog # 210-TA), Recombinant Human IL-18/ IL-1F4 stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (left-hand graph), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50C). Under these conditions, IFN-gamma secretion elicited by Recombinant Human IL-18/ IL-1F4 (40 ng/mL) is neutralized (right-hand graph) by increasing concentrations of Mouse Anti-Human IL-18 R beta /IL-1 R7 Monoclonal Antibody (Catalog # MAB1181). The ND50 is typically 0.3-1.0 µg/mL.

Western Blot Detection of Human IL-18 R beta/IL-1 R7/ACPL by Western Blot View Larger

Detection of Human IL-18 R beta/IL-1 R7/ACPL by Western Blot Soluble interleukin-18 receptor alpha complex is associated with interleukin-18 and the soluble form of the interleukin-18 receptor beta chain. (A) Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis of the serum interleukin (IL)-18 receptor alpha (IL-18R alpha ) complex. Purified H44 monoclonal antibody (mAb) was coupled to a 5-mL HiTrap NHS-activated HP column (GE Healthcare). Pooled human blood serum (120 mL) was applied to this affinity column. The IL-18R alpha complex was eluted with elution buffer at a flow rate of 2 mL/minute. Every 1 mL of the elution buffer was collected into a test tube containing 50 mL of neutralization buffer (collected fractions were denoted in order as fractions 1, 2, 3 and so on). A 10-μL aliquot of every fraction (fractions 1 to 8) was treated with the same volume of sample buffer containing 4% SDS (Tris-Glycine SDS Sample Buffer (2×); Invitrogen). Electrophoresis was carried out in the presence of 0.1% SDS, and the gel was stained with Coomassie Brilliant Blue. (B) Western blots of the serum IL-18R alpha complex using an antihuman IL-18R alpha mAb are shown. Western blot analysis was performed using antihuman IL-18R alpha mAb 70625 (R&D Systems, Inc.). Lane 1: Western blot showing 1 μg of rhIL-18R alpha /Fc chimera protein (R&D Systems, Inc.). Lane 2: Western blot showing 5 μg of isolated serum IL-18R alpha complex. (C) Western blot showing serum IL-18R alpha complex using an antihuman IL-18 mAb. Western blot analysis was performed using antihuman IL-18 mAb clone 8 with 5 μg of isolated serum IL-18R alpha complex. (D) Western blot showing serum IL-18R alpha complex using an antihuman IL-18R beta mAb. Western blot analysis was performed using antihuman IL-18R beta mAb 132016 (R&D Systems, Inc.) with 5 μg of isolated serum IL-18R alpha complex. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/21435242), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-18 R beta/IL-1 R7

IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses. The functional IL-18 receptor complex consists of two components, the IL-18 R alpha (IL-1 R5) and IL-18 R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although
IL‑18 R alpha by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R beta cDNA encodes a 599 amino acid (aa) residue precursor type I membrane protein with a 14 aa signal peptide, a 342 aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 222 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18 R beta parallels that of IL-18 R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon.

References
  1. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
  2. Okamura, H. et al. (2000) in Cytokine Reference, Vol. 2:1605, Academic Press.
Long Name
Interleukin 18 Receptor beta
Entrez Gene IDs
8807 (Human); 16174 (Mouse)
Alternate Names
Accessory protein-like; AcPL; CD218 antigen-like family member B; CD218b antigen; CD218b; CDw218b; IL-1 R7; IL-18 R beta; IL-18 receptor accessory protein; IL-18 receptor beta; IL18R beta; IL-18RAcP; IL18RAP; IL18RB; IL-18Rb; IL-18Rbeta; IL-18R-beta; IL-1R accessory protein-like; IL1R7; IL-1R7; IL-1R-7; IL-1RAcPL; interleukin 18 receptor accessory protein; Interleukin-1 receptor 7; interleukin-18 receptor accessory protein; Interleukin-18 receptor accessory protein-like; Interleukin-18 receptor beta; MGC120589; MGC120590

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Citations for Human IL-18 R beta /IL-1 R7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. The IL18RAP region disease polymorphism decreases IL-18RAP/IL-18R1/IL-1R1 expression and signaling through innate receptor-initiated pathways.
    Authors: Hedl M, Zheng S, Abraham C
    J Immunol, 2014-05-19;192(12):5924-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis.
    Authors: Takei S, Hoshino T, Matsunaga K, Sakazaki Y, Sawada M, Oda H, Takenaka S, Imaoka H, Kinoshita T, Honda S, Ida H, Fukuda TA, Aizawa H
    Arthritis Res. Ther., 2011-03-24;13(2):R52.
    Species: Human
    Sample Types: Serum
    Applications: Western Blot
  3. CD molecules 2006--human cell differentiation molecules.
    Authors: Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X
    J. Immunol. Methods, 2006-12-04;319(1):1-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. IL-18 receptor beta-induced changes in the presentation of IL-18 binding sites affect ligand binding and signal transduction.
    Authors: Wu C, Sakorafas P, Miller R, McCarthy D, Scesney S, Dixon R, Ghayur T
    J. Immunol., 2003-06-01;170(11):5571-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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