Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Neutralization

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IL-18 R beta/IL-1 R7 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-18 R beta /IL-1 R7
Phe20-Arg356
Accession # O95256

Specificity

Detects human IL‑18 R beta in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse IL‑18 R beta (AcPL), recombinant human (rh) IL-18 R, rhIL-1 R1, rhIL-1 RII, and rhIL-1 Rrp2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL‑18 R beta /IL‑1 R7 Antibody

IFN‑ gamma secretion Induced by IL‑18/IL‑1F4 and Neutralization by Human IL‑18 R beta /IL‑1 R7 Antibody.

IFN‑ gamma secretion Induced by IL‑18/IL‑1F4 and Neutralization by Human IL‑18 R beta /IL‑1 R7 Antibody.

In the presence of Recombinant Human TNF-a (20 ng/mL, Catalog # 210-TA), Recombinant Human IL-18/IL-1F4 stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN-y Quantikine ELISA Kit (Catalog # DIF50C). Under these conditions, IFN-gamma secretion elicited by Recombinant Human IL-18/IL-1F4 (40 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-18 R beta /IL-1 R7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF118). The ND50 is typically 0.3-1.2 µg/mL.

Applications for Human IL‑18 R beta /IL‑1 R7 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human IL‑18 R beta /IL‑1 R7 Fc Chimera (Catalog # 118-AP)

Neutralization

Measured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in the KG‑1 human acute myelogenous leukemia cell line. Novick, D. et al. (1999) Immunity 10(1):127. The Neutralization Dose (ND50) is typically 0.3‑1.2 µg/mL in the presence of 40 ng/mL Recombinant Human IL‑18/IL‑1F4 and 20 ng/mL Recombinant Human TNF‑ alpha.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-18 R beta/IL-1 R7

IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses. The functional IL-18 receptor complex consists of two components, the IL‑18 R alpha (IL-1 R5) and IL-18 R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although IL‑18 R alpha by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R beta cDNA encodes a 599 amino acid (aa) residue precursor type I membrane protein with a 14 aa signal peptide, a 342 aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 222 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18 R beta parallels that of IL-18 R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon.

References

  1. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
  2. Okamura, H. et al. (2000) in Cytokine Reference, Vol. 2:1605, Academic Press.

Long Name

Interleukin 18 Receptor beta

Alternate Names

AcPL, CD218b, IL-1 R7, IL-18Rb, IL18R beta, IL18RAP

Entrez Gene IDs

8807 (Human); 16174 (Mouse)

Gene Symbol

IL18RAP

UniProt

O95256

Additional IL-18 R beta/IL-1 R7 Products

Product Documents for Human IL‑18 R beta /IL‑1 R7 Antibody

Certificate of Analysis

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Product Specific Notices for Human IL‑18 R beta /IL‑1 R7 Antibody

For research use only

Citations for Human IL‑18 R beta /IL‑1 R7 Antibody

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