Human IL-28B/IFN-lambda 3 Antibody Summary
Arg30-Val200 (Lys74Arg)
Accession # AAN28264
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human IL‑28B/IFN‑ lambda 3 by Western Blot. Western blot shows lysates of human monocytes treated (+) with 10 ng/mL of rhIFN-alpha for 24 hours and then further stimulated with 10 µg/mL of poly I:C for 48 hours. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human IL-28B/IFN-lambda 3 Monoclonal Antibody (Catalog # MAB5259) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-28B/IFN-lambda 3 at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-28B/IFN-lambda 3
IL-28B (also named interferon-lambda 3, IFN-lambda 3), IL-28A (IFN-lambda 2) and IL-29 (IFN-lambda 1) are type III interferons that are class II cytokine receptor ligands (1‑4). They are distantly related to members of the IL-10 family and type I IFN family (1‑4). Human IL-28B cDNA encodes a 200 amino acid (aa) protein with a 25 aa signal peptide and a 175 aa mature protein that lacks N-glycosylation sites. Mature human IL-28B shares 64% and 75% aa sequence identity with mouse and canine IL-28B, respectively, and is active across species (5). Human IL-28B shares 94% and 69% aa identity with human IL-28A and IL-29, respectively (4). Type III interferons are widely expressed, but are mainly produced by antigen presenting cells in response to viruses and double-stranded RNA that interact with Toll-like receptors or RIG-1 family helicases (2‑6). They signal through a widely expressed receptor that is a heterodimer of the IL-10 receptor beta (IL-10 R beta ) and IL-28 receptor alpha (IL-28 R alpha ; also called IFN-lambda R1) (2, 3, 7, 9). Interaction of either type I or type III IFNs with their receptors activates similar pathways, including JAK tyrosine kinase activation, STAT phosphorylation and formation of the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex (1‑3). Both type I and III IFNs induce anti-viral activity and upregulate MHC class I antigen expression (2‑6). Cell lines responsive to type III IFNs are also responsive to type I IFNs, but in general, higher concentrations of type III IFNs are needed for similar in vitro responses (8). In vivo, however, type III IFNs enhance levels of IFN-gamma in serum, suggesting that the robust anti-viral activity of type III IFNs may stem in part from activation of the immune system (5, 7). Anti-proliferative and antitumor activity in vivo has also been shown for type III IFNs (9‑11).
- Chen, Q. et al. (2006) Vitam. Horm. 74:207.
- Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
- Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
- Bartlett, N.W. et al. (2005) J. Gen. Virol. 86:1589.
- Ank, N. et al. (2006) J. Virol. 80:4501.
- Onoguchi, K. et al. (2007) J. Biol. Chem. 282:7576.
- Siebler, J. et al. (2007) Gastroenterology 132:358.
- Meager, A. et al. (2005) Cytokine 31:109.
- Lasfar, A. et al. (2006) Cancer Res. 66:4468.
- Sato, A. et al. (2006) J. Immunol. 176:7686.
- Zitzmann, K. et al. (2006) Biochem. Biophys. Res. Commun. 344:1334.
Product Datasheets
Citations for Human IL-28B/IFN-lambda 3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Fusing the 3'UTR of seed storage protein genes leads to massive recombinant protein accumulation in seeds
Authors: Kanai, M;Sugiyama, M;Kondo, M;Yamada, K;Nishimura, M;Mano, S;
Scientific reports
Species: Plant - Arabidopsis thaliana
Sample Types: Cell Supernates
Applications: Western Blot -
Macrophage migration inhibitory factor enhances influenza-associated mortality in mice
Authors: CA Smith, DJ Tyrell, UA Kulkarni, S Wood, L Leng, RL Zemans, R Bucala, DR Goldstein
JCI Insight, 2019-07-11;4(13):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal-fetal interface
Authors: J Corry, N Arora, CA Good, Y Sadovsky, CB Coyne
Proc. Natl. Acad. Sci. U.S.A., 2017-08-07;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Hepatitis C virus pathogen associated molecular pattern (PAMP) triggers production of lambda-interferons by human plasmacytoid dendritic cells.
Authors: Stone, Amy E L, Giugliano, Silvia, Schnell, Gretja, Cheng, Linling, Leahy, Katelyn, Golden-Mason, Lucy, Gale, Michael, Rosen, Hugo R
PLoS Pathog, 2013-04-18;9(4):e1003316.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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