Human IL-36Ra/IL-1F5 Antibody

Catalog # Availability Size / Price Qty
MAB1275
MAB1275-SP
Product Details
Citations (1)
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Human IL-36Ra/IL-1F5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-36Ra/IL-1F5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-1ra, rhIL-18, rhIL-1F6, or rhIL-1F8.
Source
Monoclonal Mouse IgG2B Clone # 190524
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL-36Ra/IL-1F5
Met1-Asp155
Accession # Q9UBH0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL-36Ra/IL-1F5 (Catalog # 1275-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36Ra/IL-1F5

Human interleukin 1 family member #5 [IL-1F5; also named FIL-1δ (delta), IL-1HY1, IL-1H3, and IL-1L1] is a member of the IL-1 family of proteins (1‑5). IL-1 family members include IL-1 beta, IL-1 alpha, IL-1ra, IL-18, and IL-1F5 - IL-1F10 (6). All family members show a 12 beta -strand, beta -trefoil configuration, and all family members are believed to have arisen from a common ancestral gene that underwent multiple duplications (6). The human IL-1F5 gene is in closest proximity to the gene for IL-1ra and is likely a relatively recent duplication of the IL-1ra gene (2, 3). IL-1F5 is synthesized as a 155 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (2‑5). Nevertheless, it appears to be secreted as a 17 kDa monomer (5). There is an alternate start site that potentially gives rise to an alternate splice form (5). The translated product, however, has a premature stop codon, resulting in a truncated 16 aa peptide. Human to mouse, full length IL-1F5 has 90% aa identity. Within the family, IL-1F5 is 50% aa identical to IL-1ra, and 32%, 31%, 35%, 37%, 32%, and 42% aa identical to IL‑1 beta, IL-1F6, F7, F8, F9, and F10, respectively. Cells reported to express IL-1F5 include monocytes, B cells, dendritic cells/Langerhans cells, keratinocytes, and gastric fundus Parietal and Chief cells (1, 7). The receptor for IL-1F5 has not been positively identified. Indirect evidence suggests it is IL-1 Rrp2 and/or IL‑1 RAcP (8). In either case, activity association with receptor binding is also unclear. It was initially reported to be an antagonist of IL-1F9 activity (4, 6). This would be consistent with its hypothesized relationship to IL-1ra. Studies, however, find IL-1F5 antagonist activity difficult to demonstrate (8).

References
  1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
  2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
  3. Mulero, J.J. et al. (1999) Biochem. Biophys. Res. Commun. 263:702.
  4. Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
  5. Barton, J.L. et al. (2000) Eur. J. Immunol. 30:3299.
  6. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  7. Debets, R. et al. (2001) J. Immunol. 167:1440.
  8. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
Long Name
Interleukin 36 Receptor Antagonist/Interleukin 1 Family 5
Entrez Gene IDs
26525 (Human); 54450 (Mouse)
Alternate Names
FIL1 delta; FIL1; FIL1-delta, IL-1RP3, IL-1L1, IL-1-delta); FIL1DIL-1 delta; IL1F5 (Canonical product IL-1F5a); IL1F5; IL-1HY1; IL-1L1; IL-1RP3; IL36Ra; IL-36Ra; IL36RN; interleukin 1 family, member 5 (delta); interleukin 1, delta; Interleukin 36 Receptor Antagonist; interleukin-1 family member 5; Interleukin-1 HY1; Interleukin-1 receptor antagonist homolog 1; Interleukin-1-like protein 1; Interleukin-36 Receptor Antagonist

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Citation for Human IL-36Ra/IL-1F5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. IL-1F5, -F6, -F8, and -F9: A Novel IL-1 Family Signaling System That Is Active in Psoriasis and Promotes Keratinocyte Antimicrobial Peptide Expression.
    Authors: Johnston A, Xing X, Guzman AM, Riblett M, Loyd CM, Ward NL, Wohn C, Prens EP, Wang F, Maier LE, Kang S, Voorhees JJ, Elder JT, Gudjonsson JE
    J. Immunol., 2011-01-17;186(4):2613-22.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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