Human IL-3R alpha /CD123 Antibody

Catalog # Availability Size / Price Qty
MAB301-SP
MAB301-100
MAB301-500

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IL‑3 R alpha /CD123 in Human Peripheral Blood Lymphocytes.
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Product Details
Citations (8)
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Reviews (4)

Human IL-3R alpha /CD123 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑3 R alpha /CD123 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 32703
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑3 R alpha /CD123
Lys20-Arg305, predicted
Accession # P26951
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL‑3 R alpha /CD123 (Catalog # 301-R3) under non-reducing conditions only
Flow Cytometry
2.5 µg/106 cells
THP‑1 human acute monocytic leukemia cell line
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑3-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.6-1.2 µg/mL in the presence of 0.5 ng/mL Recombinant Human IL‑3.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry IL-3 Ra/CD123 antibody in Human Peripheral Blood Lymphocytes by Immunocytochemistry (ICC). View Larger

IL‑3 R alpha /CD123 in Human Peripheral Blood Lymphocytes. IL‑3 R alpha /CD123 was detect-ed in immersion fixed human peripheral blood lymphocytes using 2 µg/mL Mouse Anti-Human IL‑3 R alpha /CD123 Monoclonal Antibody (Catalog # MAB301) for 3 hours at room temperature. Cells were stained (red) and counter-stained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry IL-3 Ra/CD123 antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

IL‑3 R alpha /CD123 in Human Tonsil. IL-3 Ra/CD123 was detected in immersion fixed paraffin-embedded sections of human tonsil using 15 µg/mL Mouse Anti-Human IL-3 Ra/CD123 Monoclonal Antibody (Catalog # MAB301) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization Cell Proliferation Induced by IL‑3 and Neutralization by Human IL‑3 R alpha /CD123 Antibody. View Larger

Cell Proliferation Induced by IL‑3 and Neutralization by Human IL‑3 R alpha /CD123 Antibody. Recombinant Human IL-3 (Catalog # 203-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-3 Ra/CD123 Monoclonal Antibody (Catalog # MAB301). The ND50 is typically 0.6-1.2 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-3R alpha

IL-3 is a pleiotropic cytokine that can stimulate proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors (1, 2). IL-3 exerts its activity through binding to a specific cell surface receptor known as IL-3 R. IL-3 R is a heterodimeric structure composed of a 70 kDa IL-3 R alpha subunit (CD123) and a 120-140 kDa IL-3 R beta subunit (CD131) (3, 4). IL-3 R alpha binds IL-3 with relatively low affinity. In the presence of IL-3 R beta, however, IL-3 R alpha has a much higher affinity for IL-3. It is not clear how signal transduction occurs following IL-3 binding. The IL-3 R alpha chain has a very short intracellular domain while the IL‑3 R beta chain has a very large cytoplasmic domain. The IL‑3 R beta chain is also shared by the receptors for IL-5 and GM-CSF. Cells known to express IL-3 receptors include hematopoietic progenitors, epithelial cells, double negative T cells, mast cells, basophils and blood monocytes (5).

References
  1. Moore, M.A.S. et al. (1991) Blood 72:944.
  2. Warren, D.J. et al. (1988) J. Immunol. 140:94.
  3. Plant M. et al. (1989) Nature 339:150.
  4. Budel, L.M. et al. (1990) Blood 75:1439.
  5. Schrader, J.W. et al. (1988) In Interleukin-3: The Panspecific hemopoietin (ed. J.W. Schrader), Academic Press, San Diego, CA.
Long Name
Interleukin 3 Receptor alpha
Entrez Gene IDs
3563 (Human); 16188 (Mouse); 102138639 (Cynomolgus Monkey)
Alternate Names
CD123 antigen; CD123; hIL3Ra; hIL-3Ra; IL-3 R alpha; IL-3 receptor subunit alpha; IL3R alpha; IL-3R alpha; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor, alpha (low affinity); interleukin-3 receptor subunit alpha; MGC34174

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Citations for Human IL-3R alpha /CD123 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Interleukin-3-Receptor-alpha in Triple-Negative Breast Cancer (TNBC): An Additional Novel Biomarker of TNBC Aggressiveness and a Therapeutic Target
    Authors: M Koni, I Castellano, E Venturelli, A Sarcinella, T Lopatina, C Grange, M Cedrino, S Femminò, P Cossu-Rocc, S Orrù, F D'Ascenzo, I Cotellessa, C Tampieri, C Debernardi, G Cugliari, G Matullo, G Camussi, MR De Miglio, MF Brizzi
    Cancers, 2022-08-13;14(16):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Targeting IL-3R&alpha on tumor-derived endothelial cells blunts metastatic spread of triple-negative breast cancer via extracellular vesicle reprogramming
    Authors: T Lopatina, C Grange, C Cavallari, V Navarro-Ta, G Lombardo, A Rosso, M Cedrino, MAC Pomatto, M Koni, F Veneziano, I Castellano, G Camussi, MF Brizzi
    Oncogenesis, 2020-10-10;9(10):90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Myelodysplastic syndrome progression to acute myeloid leukemia at the stem cell level
    Authors: J Chen, YR Kao, D Sun, TI Todorova, D Reynolds, SR Narayanaga, C Montagna, B Will, A Verma, AU Steidl
    Nat. Med., 2018-12-03;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. A dual role for the N-terminal domain of the IL-3 receptor in cell signalling
    Authors: SE Broughton, TR Hercus, TL Nero, WL Kan, EF Barry, M Dottore, KS Cheung Tun, CJ Morton, U Dhagat, MP Hardy, NJ Wilson, MT Downton, C Schieber, TP Hughes, AF Lopez, MW Parker
    Nat Commun, 2018-01-26;9(1):386.
    Applications: Flow Cytometry
  5. Interleukin-3 enhances the migration of human mesenchymal stem cells by regulating expression of CXCR4
    Authors: A Barhanpurk, ST Mhaske, ST Pote, K Singh, MR Wani
    Stem Cell Res Ther, 2017-07-14;8(1):168.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Human Basophils Modulate Plasma Cell Differentiation and Maturation
    Authors: Almut Meyer-Bahl
    J. Immunol., 2016-11-16;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Graves' disease is associated with a defective expression of the immune regulatory molecule galectin-9 in antigen-presenting dendritic cells.
    Authors: Leskela S, Serrano A, de la Fuente H, Rodriguez-Munoz A, Ramos-Levi A, Sampedro-Nunez M, Sanchez-Madrid F, Gonzalez-Amaro R, Marazuela M
    PLoS ONE, 2015-04-16;10(4):e0123938.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte macrophage-colony-stimulating factor.
    Authors: Bovolenta C, Pilotti E, Mauri M, Turci M, Ciancianaini P, Fisicaro P, Bertazzoni U, Poli G, Casoli C
    Blood, 2002-01-01;99(1):224-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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Reviews for Human IL-3R alpha /CD123 Antibody

Average Rating: 4 (Based on 4 Reviews)

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Human IL-3R alpha /CD123 Antibody
By Anonymous on 06/10/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human IL-3 R alpha /CD123 Antibody
By Anonymous on 11/20/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Human IL-3 R alpha /CD123 Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

By Mehdi Alavi on 03/28/2018
Application: IHC Sample Tested: DX-3 human melanoma cell line Species: Human