Human IL-4R alpha Antibody

Catalog #: AB-230-NA Datasheet / COA / SDS
Catalog # Availability Size / Price Qty

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Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 R alpha  Antibody.
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Human IL-4R alpha Antibody Summary

Species Reactivity
Detects human IL‑4 R alpha  in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) IL‑5 R alpha, rhIL-5 R beta, rhIL-9 R, rhIL-13 R alpha 1, rhIL-13 R alpha 2 and recombinant mouse IL-4 R is observed.
Polyclonal Goat IgG
Protein A or G purified
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑4 R alpha
Accession # P24394
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human IL‑4 R alpha (Catalog # 230-4R)
Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 5-10 µg/mL in the presence of 0.2 ng/mL Recombinant Human IL‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 R alpha  Antibody. View Larger

Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 R alpha Antibody. Recombinant Human IL-4 (Catalog # 204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-4 (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-4 Ra Polyclonal Antibody (Catalog # AB-230-NA). The ND50 is typically 5-10 µg/mL.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 1 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4R alpha

Interleukin 4 (IL-4) is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. The biological functions of IL-4 are mediated by the binding of IL‑4 to high-affinity cell surface receptor complexes. Two types of IL-4 receptor complexes have been described. The type I IL-4 receptor complex is composed of a high-affinity IL-4-binding subunit (referred to as IL-4 R alpha ) and the common gamma chain that does not bind IL-4 by itself. The type II IL-4 receptor complex is composed of IL-4 R alpha and IL-13 R alpha 1. Besides IL-4 signals, the type II IL-4 receptor complex can also transduce IL-13 signals. In the type II complex, the IL-4 R alpha subunit binds only IL-4 and not IL-13. Similarly, the IL-13 R alpha 1 subunit binds only IL-13 and not IL-4. The cDNA clones for both the human and mouse IL-4 R alpha have been isolated and shown to encode an approximately 140 kDa type I transmembrane protein with a large cytoplasmic domain that is essential for signal transduction. In mouse cells, an alternatively spliced variant encoding a soluble secreted IL-4 R alpha isoform has also been identified. Naturally occurring soluble IL-4 R alpha that binds IL-4 with high-affinity has been found in mouse and human biological fluids.

  1. Keegan, A.D. (2001) in Cytokine Reference, Academic Press, Vol. 1:127.
Long Name
Interleukin 4 Receptor alpha
Entrez Gene IDs
3566 (Human); 16190 (Mouse); 102133649 (Cynomolgus Monkey)
Alternate Names
CD124 antigen; CD124; IL-4 R alpha; IL-4 receptor subunit alpha; IL4R alpha; IL-4R alpha; IL-4R subunit alpha; IL4R; IL-4Ra; IL4RACD124; IL-4R-alpha; interleukin 4 receptor; interleukin-4 receptor alpha chain; interleukin-4 receptor subunit alpha

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